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T9179

Sigma-Aldrich

TB培养基

EZMix powder microbial growth medium

同義詞:

TB media

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About This Item

分類程式碼代碼:
41106200
NACRES:
NA.85
暫時無法取得訂價和供貨情況

等級

Molecular Biology

品質等級

200

描述

TB; quick dissolve, dust-free formulation

無菌

non-sterile

形狀

powder

技術

microbiological culture: suitable

應用

agriculture
food and beverages
microbiology

儲存溫度

room temp

適合性

nonselective for Escherichia coli
nonselective for coliforms

相關類別

一般說明

Terrific Broth是一个被高度引用的用于大肠杆菌培养的微生物生长培养基。 这种营养丰富的微生物肉汤含有甘油、肽、氨基酸、水溶性维生素和碳水化合物。

應用

适用于大肠杆菌菌株的非选择性培养，用于克隆、高产量生产 DNA 质粒和高产量生产重组蛋白。当添加适当的抗生素时，也适用于选择性培养。
使用了很棒的肉汤：
  • 作为在 pET28GST-LIC 中选择性生长表达 MLL SET（核原癌基因）结构域的大肠杆菌 BL21（DE3） V2RpRARE 的培养基[1]
  • 培养含有表达 IL-8（interleukin-8） 质粒的 BL21（DE3） 细胞[2]
  • 很棒的肉汤也适用于细菌悬浮液（枯草芽孢杆菌菌株 1085B）[3]

特點和優勢

很棒的 Broth EZMix 粉末提供：
  • 无尘颗粒，便于安全操作和更快混合
  • 一种预算较低的液体替代品
  • 改良配方（不含甘油）
  • 可通过大包装轻松放大规模

成分

*请注意，某些产品规格以液体形式提供。 本品为粉末形式，并且这些规格反映了最终的重悬体积。

23.6g/L酵母提取物
11.8g/L 胰蛋白胨
9.4g/L K2HPO4
2.2g/L KH2PO4

重構

将 24.1 g 粉末和 4 mL 甘油悬浮在足量的去离子水中，制成 500 mL 溶液。 在121 °C下高温灭菌15分钟以灭菌。 先冷却再添加其他成分，如抗生素（如果需要）。

法律資訊

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000417640
0000417640
0000417645
0000417645
0000374377

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存取文件庫

Lac V Lee et al.
Biochemistry, 49(25), 5366-5376 (2010-05-19)
Undecaprenyl pyrophosphate synthase (UPPS) catalyzes the consecutive condensation of eight molecules of isopentenyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to generate the C(55) undecaprenyl pyrophosphate (UPP). It has been demonstrated that tetramic acids (TAs) are selective and potent inhibitors of
Cynthia Sanville Millard et al.
Protein expression and purification, 29(2), 311-320 (2003-05-28)
Contemporary approaches to biology often call for the high-throughput production of large amounts of numerous proteins for structural or functional studies. Even with the highly efficient protein expression systems developed in Escherichia coli, production of these proteins is laborious and
Paul Peluso et al.
Analytical biochemistry, 312(2), 113-124 (2003-01-18)
Antibody microarrays have the potential to revolutionize protein expression profiling. The intensity of specific signal produced on a feature of such an array is related to the amount of analyte that is captured from the biological mixture by the immobilized
Eve Toulza et al.
Applied and environmental microbiology, 76(13), 4521-4529 (2010-05-04)
Understanding the conditions leading to harmful algal blooms, especially those produced by toxic dinoflagellate species, is important for environmental and health safety. In addition to investigations into the environmental conditions necessary for the formation of toxic blooms, we postulate that
Optimizing antibody immobilization strategies for the construction of protein microarrays
Peluso P, et al.
Analytical Biochemistry, 312(2), 113-124 (2003)
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條款

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

微生物生長協議

培養大腸桿菌的規模從小培養到發酵。包括啟動培養、懸浮細胞、單菌培養和噬菌體 M13 複製的程序。

Questions

1–2 of 2 Questions  

  1. Which bacterial culture medium is the best choice for my application?

    1 answer

    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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