L7533

LB Broth with agar (Lennox)

EZMix powder microbial growth medium

• 同義詞: LB broth
• 分類程式碼代碼: 41106200
• NACRES: NA.85

屬性

• 等級: for molecular biology
• 品質等級: 200
• 無菌: non-sterile
• 形狀: powder
• 成份: Agar, 15 g/L ; NaCl, 5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• 技術: microbiological culture: suitable
• 應用: agriculture; food and beverages; microbiology
• 儲存溫度: room temp
• 適合性: nonselective for Escherichia coli; nonselective for coliforms

描述

一般說明

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

應用

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

特點和優勢

Lennox EZMix powder with agar provides:

• A budget-friendly alternative to pre-poured plates
• Granulated, dust-free format for safer handling and faster mixing
• Convenient small package to eliminate weighing
• Easy scale-up using larger package sizes
• Standard formulation

準備報告

1. Suspend 35.6 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121°C.
To prepare Lennox L Agar: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.

重構

Add 35.6g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

法律資訊

Zeocin is a registered trademark of Cayla Sarl

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable