The formulation for L3022 has not changed over the years. The "low salt" label was added later to help customers identify the formulation more easily.
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等級
Molecular Biology
品質等級
無菌
non-sterile
形狀
powder
成份
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
技術
microbiological culture: suitable
pH值
6.8-7.2(2% solution)
應用
food and beverages
microbiology
儲存溫度
room temp
適合性
nonselective for Escherichia coli
nonselective for coliforms
一般說明
Lennox LB是一个被高度引用的用于 大肠杆菌培养的微生物生长培养基。这种富含营养的微生物肉汤含有肽、氨基酸和低盐配方的碳水化合物。
應用
Luria肉汤被用于大肠杆菌（Escherichia coli）的维持和繁殖。 大肠杆菌在Luria肉汤中生长得更快，因为胰蛋白胨和酵母为其提供了必需的生长因子，从而无需大肠杆菌自身合成。由于含有NaCl成分，Luria肉汤还含有转运和渗透平衡所必需的电解质。[1]
在三鞭毛虫细胞提取物的分级研究中，它被用于梨形四膜虫胞级分的重悬。[2]
在三鞭毛虫细胞提取物的分级研究中，它被用于梨形四膜虫胞级分的重悬。[2]
适用于 大肠杆菌 菌株的非选择性培养，用于克隆、生产DNA质粒和生产重组蛋白。 也适用于添加适当抗生素时的选择性培养，包括需要低盐条件的抗生素，如吉欧霉素®。
特點和優勢
Lennox LB粉末可提供：
- 便捷式小包装，无需称重步骤
- 使用大包装可轻松放大规模
- 一种预算较低的液体替代品
- 标准配方
準備報告
1.将20 g悬浮于1L蒸馏水中。
2.121 °C下高压灭菌15分钟。
制备LB肉汤：添加1 g葡萄糖，然后按照上述说明操作。
制备Enquist &Sternberg培养基：高压蒸汽灭菌后，以无菌操作加入10 mL无菌1M硫酸镁。
2.121 °C下高压灭菌15分钟。
制备LB肉汤：添加1 g葡萄糖，然后按照上述说明操作。
制备Enquist &Sternberg培养基：高压蒸汽灭菌后，以无菌操作加入10 mL无菌1M硫酸镁。
法律資訊
Zeocin is a registered trademark of Cayla Sarl
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產品號碼
描述
訂價
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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General protocols for growth of competent cells in microbial medium.
培養大腸桿菌的規模從小培養到發酵。包括啟動培養、懸浮細胞、單菌培養和噬菌體 M13 複製的程序。
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Are the new batches of the product labeled as "Low salt" consistent with the older batch that does not have the same label? Consistency in experiments is a concern.
1 answer-
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What are the differences among the Luria, Lennox and Miller LB formulations?
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LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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Will adding magnesium to the culture medium increase cell density?
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In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What is the difference among the many LB - Lennox products?
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LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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