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Merck
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T2694

Sigma-Aldrich

Trizma® 盐酸溶液

pH 8.0, BioPerformance Certified, 1 M, suitable for cell culture

同義詞:

Tris 盐酸盐溶液

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

等級

BioPerformance Certified
for molecular biology

品質等級

形狀

solution

濃度

1 M

技術

cell culture | mammalian: suitable

雜質

DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH值

8.0

有用的pH值範圍

7.0-9.0

吸收

≤0.05 at 290 at 40%

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一般說明

一系列 TRIZMA 碱和 TRIZMA HCl 的预混合溶液,为 Tris 缓冲液提供常用的 pH 值。不必混合或调节 pH 值。保证精确度 ± 0.1 个 pH 单位。

應用

Trizma® 盐酸溶液用于:
  • 作为提取缓冲液成分,分离甘蓝根肿菌 DNA
  • 作为重悬缓冲液成分,用于人类基因组DNA片段样品
  • 调节血浆样品pH值,作为重组和中和缓冲液成分

法律資訊

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


分析證明 (COA)

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Ultra-deep targeted sequencing (UDT-Seq) can identify subclonal somatic mutations in tumor samples. Early assays' limited breadth and depth restrict their clinical utility. Here, we target 71 kb of mutational hotspots in 42 cancer genes. We present novel methods enhancing both
Accurate variant detection across non-amplified and whole genome amplified DNA using targeted next generation sequencing
ElSharawy A, et al.
BMC Genomics, 13(1), 500-500 (2012)
Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae
Mehrabi S, et al.
Bio-protocol (2018)
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