This product is tested under both unreduced and reduced conditions. Under unreduced conditions, the SDS-PAGE gel shows a band at 26 kDa, while under reduced conditions, the only band is around 13 kDa molecular weight.
推薦產品
生物源
human
重組細胞
expressed in E. coli
化驗
≥97% (HPLC)
≥97% (SDS-PAGE)
形狀
lyophilized
效力
≤2.0 ng/mL ED50
分子量
26.0 kDa
包裝
pkg of 10 μg
技術
cell culture | mammalian: suitable
雜質
<0.1 EU/μg endotoxin, tested
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... INHBA(3624)
mouse ... Inhba(16323)
rat ... Inhba(29200)
一般說明
Activins (also referred to as inhibins) are generally dimers of three subunits: inhibin βA, inhibin βB, and inhibin α.[1] Recombinant human Activin A (inhibin βA) is a 26kDa disulfide-linked homodimer of two bA chains, each containing 116 amino acid residues. The sequence of mature Activin A is completely homologous between human, mouse, and rat species.
應用
Activin A has been used in monolayer induction for endoderm (hepatocyte) differentiation.[2]
Activin A human has been used to study rRNA gene transcription during directed differentiation of human embryonic stem cells.[3]
生化／生理作用
Activin A (inhibin βA) is a TGF (transforming growth factor)-β family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival.[4] Elevated levels of Activin A in human colorectal tumors and in postmenopausal women have been implicated in colorectal and breast cancers, respectively.[1][5] Activin A plays a central role in reproduction by regulating the levels of follicle stimulating hormone (FSH). It is involved in hematopoiesis, embryogenesis and osteogenesis. It exhibits both tumor suppressing and promoting effects.[6] In underweight and overweight humans, high levels of Activin A in serum might be associated with the pathogenesis of asthma.[7] The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-β antagonist, follistatin.[8]
序列
GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS
外觀
Lyophilized with no additives.
重構
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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Mechanisms of TGF-beta signaling from cell membrane to the nucleus.
Shi Y and Massague J
Cell, 113, 685-700 (2003)
Generation of an induced pluripotent stem cell line from chorionic villi of a Turner syndrome spontaneous abortion.
Parveen S
Stem Cell Research, 12-16 (2017)
The Regulation of rRNA Gene Transcription during Directed Differentiation of Human Embryonic Stem Cells.
Woolnough JL, et al.
PLoS ONE, 11, e0157276-e0157276 (2016)
Activin A is associated with asthma in underweight and overweight patients.
Zhang LL and Liu CT
Genetics and molecular research : GMR, 14, 440-452 (2015)
Serum and tissue expression of activin a in postmenopausal women with breast cancer.
Reis FM, et al.
The Journal of Clinical Endocrinology and Metabolism, 87, 2277-2282 (2002)
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How does this protein appear on a non-reducing SDS-PAGE? Should it be present in its monomeric or its dimeric form? Or does it occur in variable ratios of the two? Does the amount of protein applied, influence its banding pattern?
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