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重要文件

A9165

Sigma-Aldrich

N-乙酰基-L-半胱氨酸

N-Acetyl-L-cysteine

≥99% (TLC), suitable for cell culture, BioReagent

同義詞:

LNAC, NAC

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About This Item

線性公式:
HSCH2CH(NHCOCH3)CO2H
CAS號碼:
分子量::
163.19
Beilstein:
1724426
EC號碼:
MDL號碼:
分類程式碼代碼:
12352209
PubChem物質ID:
NACRES:
NA.26
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產品名稱

N-乙酰基-L-半胱氨酸, BioReagent, suitable for cell culture

產品線

BioReagent

品質等級

化驗

≥99% (TLC)

形狀

powder

技術

cell culture | mammalian: suitable

顏色

white to off-white

mp

106-108 °C (lit.)

溶解度

H2O: 100 mg/mL (with heating)

儲存溫度

2-8°C

SMILES 字串

CC(=O)N[C@@H](CS)C(O)=O

InChI

1S/C5H9NO3S/c1-3(7)6-4(2-10)5(8)9/h4,10H,2H2,1H3,(H,6,7)(H,8,9)/t4-/m0/s1

InChI 密鑰

PWKSKIMOESPYIA-BYPYZUCNSA-N

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應用

N-乙酰-L-半胱氨酸已被用作小鼠肠道干细胞培养的肠道基础培养基的组成部分,也被用作膨胀培养基的组成部分。[1][2]

生化/生理作用

抗氧化剂和黏液溶解剂(mucolytic agent)。增加细胞池的自由基清除剂。 据报道可防止神经细胞凋亡,但会诱导平滑肌细胞凋亡。抑制HIV复制。可作为微粒体谷胱甘肽转移酶的底物。

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產品號碼
描述
訂價

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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存取文件庫

Derivation and long-term expansion of human endometrial and decidual organoids
Margherita Turco
Protocol Exchange, 2639-2644 (2017)
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells
Kevin P. O?Rourke
Bio-protocol, 6(4), e1733-e1733 (2016)
Meritxell Huch et al.
Nature, 494(7436), 247-250 (2013-01-29)
The Wnt target gene Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) marks actively dividing stem cells in Wnt-driven, self-renewing tissues such as small intestine and colon, stomach and hair follicles. A three-dimensional culture system allows long-term clonal expansion of single Lgr5(+) stem
Suzanne M Cloonan et al.
Journal of medicinal chemistry, 58(11), 4494-4505 (2015-05-12)
Ruthenium polypyridyl complexes show great promise as new photodynamic therapy (PDT) agents. However, a lack of detailed understanding of their mode of action in cells poses a challenge to their development. We have designed a new Ru(II) PDT candidate that
Yuval Samuni et al.
Biochimica et biophysica acta, 1830(8), 4117-4129 (2013-04-27)
N-acetylcysteine (NAC) has been in clinical practice for several decades. It has been used as a mucolytic agent and for the treatment of numerous disorders including paracetamol intoxication, doxorubicin cardiotoxicity, ischemia-reperfusion cardiac injury, acute respiratory distress syndrome, bronchitis, chemotherapy-induced toxicity

文章

Importance and uses of cysteine in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

半胱氨酸在無血清真核細胞(包括雜交瘤和中國倉鼠卵巢 (CHO) 細胞)培養中的重要性和用途

Human pancreatic cancer organoid biobank (PDAC organoids) with various KRAS mutations to aide in 3D cell culture and cancer research applications.

Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily.

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相關內容

Monitor barrier formation using colon PDOs, iPSC-derived colon organoids, Millicell® cell culture inserts, and the Millicell® ERS. 3.0.

Chromatograms

application for HPLC

Questions

1–10 of 12 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. Is this product sterile? If not, can I pass it through a 0.22 um filter unit?

    1 answer
    1. This product is not supplied sterile. However, if the water used is ultrapure, cell culture grade, and prepared in a sterile environment, there is no need to filter the solution.

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  4. Can aliquots be freeze-thawed more than once?

    1 answer
    1. Product A9165 recommended storage is 2-8°C, Regrettably, information about stability after the material is reconstituted is unavailable. Generally speaking, avoid repeated freeze-thawed.

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  5. Can aliquots be stored long term at -80 Celsius?

    1 answer
    1. As mentioned in the previous Q&A, it is recommended to store the aliquoted solutions at -20 °C for up to 1 month. Stability testing at -80°C has not been performed. It is not known if lower storage temperatures will extend the stability of the solution.

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  6. How can I make 1M N-acyteyl cysteine solution? Should I dilute in PBS or distilled water?

    1 answer
    1. This product can be dissolved in H2O up to 100 mg/mL (with heating) . PBS can be used as well. Aliquot and store solutions at -20 deg. C up to 1 month.

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  7. What is the molecular weight of product A9165, N-Acetyl-L-cysteine (NAC)?

    1 answer
    1. As listed on the product detail page, the molecular weight is determined as 163.19

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  8. How stable is a solution of Product A9165, N-acetyl cysteine (NAC)?

    1 answer
    1. Aqueous solutions of cysteine oxidize to cystine on contact with air at neutral or alkaline pH. We recommend making aqueous solutions fresh.  NAC is relatively stable in acid.  Solutions in acid are stable and can be stored at -20 °C.

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  9. What is the half life of Product A9165, N-Acetyl-L-cysteine (NAC), in cell culture?

    1 answer
    1. We have not tested the half-life of NAC solutions in our labs here at Sigma. However, this subject has been reviewed in Neuropsychopharmacology, 29, 2018-2023 (2004).

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  10. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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1–10 of 12 Questions  

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我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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