If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
推薦產品
生物源
Porcine intestinal mucosa
品質等級
種類
Grade I-A
產品線
BioReagent
形狀
powder
比活性
≥180 USP units/mg
技術
analytical sample preparation: suitable
cell culture | mammalian: suitable
顏色
beige
溶解度
H2O: 50 mg/mL
應用
cell analysis
diagnostic assay manufacturing
life science and biopharma
sample preparation
SMILES 字串
[Na+].[S](=O)(=O)(NC1C(OC(C(C1O[S](=O)(=O)O)OC4OC(C(C(C4O[S](=O)(=O)O)O)O)C(=O)O)CO)OC2C(OC(C(C2O)O[S](=O)(=O)O)OC3C(OC(C(C3O)NC(=O)C)O)CO[S](=O)(=O)O)C(=O)O)O
InChI
1S/C26H42N2O37S5.Na/c1-4(30)27-7-9(31)13(6(56-23(7)39)3-55-67(43,44)45)58-26-19(65-70(52,53)54)12(34)16(20(62-26)22(37)38)60-24-8(28-66(40,41)42)15(63-68(46,47)48)14(5(2-29)57-24)59-25-18(64-69(49,50)51)11(33)10(32)17(61-25)21(35)36;/h5-20,23-26,28-29,31-
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相關類別
一般說明
應用
特點和優勢
- 高纯度化合物适用于各种研究应用
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
從最近期的版本中選擇一個：
分析證明 (COA)
客戶也查看了
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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H3149-10KU heparin sodium salt is steril??
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The product is not sterile. Prepared solutions may be filter sterilized.
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please, could you explain me if this H3149 is one of 'high molecolar weight heparin'? thanks
1 answer-
This product is a cell culture tested porcine heparin with a molecular weight range of 15-19 kDa. This material is considered a "high molecular weight heparin." According to the literaure, low molecular weight heparins are those with a molecular wieght of less that 8000 Da. See the link below to review a publication that may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10141002/Helpful?
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Can this material be diluted to 10,000 U/mL and stored at 2C to 8C? Do you have stability data at this temperature for lower concentrations?
1 answer-
Yes, this product remains biologically active in dilute solutions. Stock solutions prepared at 50 mg/mL in water are stable for up to 2 years at 2 - 8°C, when filter sterilized. According to literature, heparin diluted in physiological saline at a concentration of 10 to 100 units/mL is stable for at least 35 days when stored at 2 - 8°C. Please see the link below to review the datasheet for an identical product, H3393, which may be applied to this item
H3393 Datasheet
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/112/608/h3393pis.pdfHelpful?
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Is the heparin sodium salt (H3149, CAS 9041-08-1) unfractionated or fractionated? How does this classification relate to its molecular weight?"
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The heparin sodium salt (H3149, CAS 9041-08-1) is unfractionated. This classification is reflected in its molecular weight, which ranges approximately from 9,000 to 21,000, with the majority falling between 17,000-19,000. As confirmed by our product and as indicated in the USP monograph, our heparin is unfractionated. This differs from fractionated Heparin, also known as Low Molecular Weight Heparin, which has a much lower molecular weight. According to the DBR, 3rd ed., p. 342, the molecular weight of our heparin is approximately 16,000-17,000.
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Can you recommend a suitable heparin product for mouse heart perfusion?
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Our available heparin products have not undergone specific testing for mouse heart perfusion. However, you may find H3149 of interest as it has been cited in the following publication for this intended application: "Isolation and Primary Culture of Mouse Aortic Endothelial Cells" published in J Vis Exp. 2016; (118): 52965 (PMID: 28060318).
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Is it a different substance from heparan sulfate?
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Heparin is not equivalent to heparin sulfate. Please see the link below to describe the differences:
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/what-is-heparinHelpful?
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Can I dissolve the heparin in filtered (0.22 um) nuclease free water at a concentration of 50mg/ml? Does it correspond to 5000 IU/ml? Then, I can use 15-30 IU/ ml of blood to avoid its coagulation, is it correct?
1 answer-
As mentioned on the Product Detail Page, this product is soluble at 50 mg/mL in water. The actual activity is reported on the lot-specific Certificate of Analysis. This value should be used in determining the activity of the prepared solution. The activity specification for this product is >/=180 USP units/mg. For example, assuming an activity value of 200 units/mg, a prepared solution of 50 mg/ml will have an activity of 5000 units/ml. Yes, typically, 15 - 30 units/ml is suitable for use as an anticoagulant.
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Can we store the solution of Heparin sodium from intestinal mucosa of pig in RNase free water at -25 C?
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Solutions can be stored at 2-8 °C for up to 2 years, if sterile filtered through a 0.22 micron filter. The freeze/thaw process may cause degradation of the polysaccharide.
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