SHP002

CRISPR & MISSION^® Lentiviral Packaging Mix

• 同義詞: Lentiviral Packaging Mix
• 分類程式碼代碼: 41106609
• NACRES: NA.51

屬性

• 濃度: 100 ng/μL (in Tris-EDTA)
• 品質等級: 200
• 應用: CRISPR
• 運輸包裝: dry ice
• 儲存溫度: −20°C

描述

一般說明

The CRISPR & MISSION Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene.

Lentiviral particles are generated from three main components:

• The packaging vector, which contains the minimal set of lentiviral genes required to generate the virion structural proteins and packaging functions.
• The vesicular stomatitis virus G-protein envelope vector, which provides the heterologous envelope for pseudotyping and allows these lentiviral particles to efficiently deliver the transfer sequence of interest to a wide variety of cell types, including primary and non-dividing cells
• The lentiviral transfer vector, which contains the sequence of interest as well as the cis acting sequences necessary for packaging.

The Lentiviral Packaging Mix contains the first two components; it is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. This product is recommended for packaging Lenti CRISPR, Lenti ORF, and Lenti shRNA vectors.

應用

功能性基因组学研究/筛选/靶标验证

法律資訊

CRISPR Use License Agreement

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable