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一般說明
MISSION ® shRNA 产品线以针对注释小鼠和人类基因的基于转移载体的 RNAi 文库为中心，可与这种慢病毒包装组合配合使用。在该包装系统中，VSV-G 的伪分型拓宽了与病毒相关的病毒嗜性。[1]因此，这些慢病毒颗粒可以高效地将感兴趣的转移序列传递给各种各样的细胞类型，包括原代和非分裂细胞。[2][3]
MISSION® 慢病毒包装混合物是由表达关键 HIV 包装基因和异源病毒包膜基因的两个质粒的优化配方。
慢病毒颗粒由三个主要组分产生：
慢病毒包装混合物包含前两种成分；它被设计为与兼容的慢病毒转移载体共转染，顺序产生用于下游转导应用的高滴度假型慢病毒颗粒。[4]
MISSION® 慢病毒包装混合物是由表达关键 HIV 包装基因和异源病毒包膜基因的两个质粒的优化配方。
慢病毒颗粒由三个主要组分产生：
- 包装载体，其中包含了生成病毒体结构蛋白和包装功能所需的最低限度的慢病毒基因集。
- 水疱性口炎病毒 G 蛋白包膜载体，为假型提供异源包膜。
- shRNA 转移载体，其中包含目的序列以及 RNA 生产和包装所必需的顺式作用序列。
慢病毒包装混合物包含前两种成分；它被设计为与兼容的慢病毒转移载体共转染，顺序产生用于下游转导应用的高滴度假型慢病毒颗粒。[4]
其他說明
法律資訊
使用本产品须遵守一项或多项许可证协议。详情请见http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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文章
MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
當使用慢病毒載體傳遞 shRNA 時，編碼 shRNA 的序列會整合到基因組中，而基因敲除的效果會傳給子細胞，繼續基因沉默。
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Preparation of the Lentiviral Transduction Particles Using Packaging Plasmid Mix
在此包裝系統中，與 VSV-G 的偽型擴大了與病毒相關的病毒滋養性。然後，慢病毒顆粒可將序列傳遞至各種細胞類型，包括原代細胞和非分裂細胞。
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