Yes, this material has a fluorescence spectrum similar to DAPI, with excitation at 340-380 nm and emission at 385-470 nm. The images displayed above were acquired using UV filter set (340-380 nm excitation, 40 nm dichroic, 430-nm long pass filter). Please note that Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately.
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一般說明
Filipin III是一种多烯大环内酯类抗生素，是从S. filipinensis培养物中分离出来的主要成分。1 Filipin因其能够发出荧光并与胆固醇结合的能力而被广泛
用作生物膜中甾醇定位的探针 2，以及在C型Niemann-Pick病的研究中，在临床上用作游离胆固醇的染色剂。 3 Filipin可抑制朊病毒蛋白（PrP）内吞作用并导致PrP从质膜释放。 4 Filipin III被发现可在烟草细胞中引发信号反应，包括NADPH氧化酶依赖性产生。5。其抗真菌作用机制可能是通过与膜甾醇结合而改变膜通透性和相关功能。Filipin可抑制朊病毒蛋白（PrP）内吞作用并导致PrP从质膜释放。
用作生物膜中甾醇定位的探针 2，以及在C型Niemann-Pick病的研究中，在临床上用作游离胆固醇的染色剂。 3 Filipin可抑制朊病毒蛋白（PrP）内吞作用并导致PrP从质膜释放。 4 Filipin III被发现可在烟草细胞中引发信号反应，包括NADPH氧化酶依赖性产生。5。其抗真菌作用机制可能是通过与膜甾醇结合而改变膜通透性和相关功能。Filipin可抑制朊病毒蛋白（PrP）内吞作用并导致PrP从质膜释放。
應用
Filipin已在双染色操作中作为探针，用于检测聚丙烯酰胺凝胶中的脂蛋白并将其固定在硝酸纤维素膜上。它还广泛用于通过特定的荧光复合物对未酯化的胆固醇进行定位和定量。
其他說明
- Filipin III溶液对空气和光非常敏感。收到产品后请进行等分并在-20°C条件下储存，避免循环冻融。
- Filipin III可多种浓度进行使用，其取决于所使用的具体方案。根据所使用的方案，可用合适的缓冲液对1mg/ml的储备溶液进行稀释。
- Filipin III与胆固醇的相互作用改变了吸收和荧光光谱，因此在用荧光显微镜观察时，使用340-380nm作为激发波长，385-470nm作为发射波长（Filipin荧光染色光漂白速度非常快，因此应立即对样品进行分析） 。
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
188.6 °F
閃點（°C）
87 °C
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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Would it be possible to image this product using a DAPI filter (Excitation 377 nm, Emission 447 nm)?
1 answer-
Helpful?
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Any suggestions for addressing white precipitations observed during Filipin staining for endothelial cells, possibly due to DMSO? Also, seeking advice to improve the experiment where most cells were negative for Filipin staining, and only a few were positive while measuring cholesterol content in HUVEC cells.
1 answer-
To address white precipitations observed during Filipin staining for endothelial cells, increasing the amount of DMSO in the final staining solution may be necessary. This can be achieved by diluting the 1 mg/ml Filipin III solution with more DMSO solution to create a 0.5 mg/ml or 0.1 mg/ml concentrated solution prior to staining the samples.
For improving cholesterol content measurement in HUVEC cells, optimize the working concentration of Filipin and conduct a time-course experiment to enhance staining results. Prompt analysis of stained samples can help prevent photobleaching.Helpful?
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