The Assay/Sample Diluent Buffer (Item E2) should be diluted 5-fold. This 1X Assay/Sample Diluent Buffer is used to dilute serum, plasma, or medium samples a recommended 2 fold. Please note that the levels of IL-6 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator.
推薦產品
物種活性
mouse
包裝
kit of 96 wells (12 strips x 8 wells)
技術
ELISA: suitable
capture ELISA: suitable
輸入
sample type plasma
sample type cell culture supernatant(s)
sample type serum
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 0.82-600 pg/mL
檢測方法
colorimetric
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
mouse ... Il6(24498)
一般說明
小鼠 IL-6 ELISA（酶联免疫吸附试验）试剂盒是一种体外酶联免疫吸附试验，用于定量测定血清、血浆、细胞培养上清液和尿液中的小鼠 IL-6。
免疫原
重组小鼠 IL-6
生化／生理作用
IL-6 是一种多效性细胞因子，通过调节免疫和炎症反应在宿主防御中发挥重要作用。IL-6 由 T 细胞、单核细胞、成纤维细胞、内皮细胞和角质形成细胞产生，具有多种生物学功能。刺激 B 细胞分化和抗体生成，与 IL-3 协同作用于巨核细胞发育和血小板生成，诱导肝脏急性期蛋白表达，调节骨代谢。[4]
其他說明
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訊號詞
Warning
危險聲明
防範說明
危險分類
Met. Corr. 1
儲存類別代碼
8A - Combustible corrosive hazardous materials
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Hello. How much should I dilute tissue culture media for the assay? 2-fold? Thanks.
1 answer-
Helpful?
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マウスのパイエル板をホモジナイズした上清で測定は可能ですか？
1 answer-
This product is suitable with serum, plasma, and cell culture supernatant. However, it has not been determined if this product can measure the supernatant obtained by homogenizing mouse Peyer's patches. This application will need to be validated by the end-user.
While the requested ELISA kit nor any of the offered normal ELISA kits are neither designed nor validated with cell/tissue lysate samples, our kits are very accommodating to many different sample types so lysates will most likely work. If it is decided to test these kits with cell/tissue lysate samples, it is recommended to dilute the samples at least 5-fold with Assay Diluent to minimize any effects of the detergents in the lysis buffer. The samples may need to be diluted further but this would need to be determined by the experimenter empirically.
So for the original lysate, in general it is recommended to shoot for at least 1 mg/ml protein, preferably more, to achieve 50-500 ug/ml after dilution. Again though, this range should just be used as a starting point and the optimal concentration would need to be determined empirically.
Below are some general guidelines for doing so along with some basic tips for sample preparation.
Tips on Sample preparation
In brief, a lysis buffer must meet the following specifications:
A) has relatively low salt content (700 mM or less)
B) does not contain sodium azide
C) does not contain >0.1% SDS
D) does not contain >10 mM reducing agents (beta-mercaptoethanol or dithiothreitol)
This would include any buffers used for immunoprecipitations, including RIPA buffer.Helpful?
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