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重要文件

R7638

Sigma-Aldrich

RPMI-1640 培养基

Dutch Modification, with sodium bicarbonate and HEPES, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

同義詞:

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

產品名稱

RPMI-1640 培养基, Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

pH值

>7.2

成分

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。

應用

RPMI-1640 Medium has been used

  • to obtain spleen and lymph nodes from mice
  • for the resuspension of Staphylococcus aureus strains in the phagocytosis assay
  • in the isolation of hepatitis B surface antigen-specific B-cell clones

重構

Supplement with 0.3 g/L L-glutamine.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Vitória M Silva et al.
BMC veterinary research, 17(1), 282-282 (2021-08-27)
The present study explored the viability of bovine milk macrophages, their intracellular production of reactive oxygen and nitrogen species (RONS), and their phagocytosis of Staphylococcus aureus, as well as the profile of lymphocytes, from healthy udder quarters and udder quarters
Kamila R Santos et al.
Vaccines, 9(8) (2021-08-29)
Staphylococcus aureus mastitis remains a major challenge for dairy farming. Here, 24 mice were immunized and divided into four groups: G1: control; G2: Granulocyte Macrophage Colony-Stimulating Factor (GM-CSF) DNA vaccine; G3: F0F1 ATP synthase subunit α (SAS), succinyl-diaminopimelate (SDD), and
Blood polymorphonuclear leukocyte responses against Staphylococcus aureus in primiparous and pluriparous Lacaune and Santa Ines ewes
Marques M, et al.
Small Ruminant Research, 201, 106412-106412 (2021)
Ewerton de Souza Lima et al.
Frontiers in veterinary science, 8, 650021-650021 (2021-07-06)
The implications of bovine leukemia virus (BLV) on innate and adaptive immune responses have been widely investigated; however, the effects of BLV on mammary gland immunity require further investigation. The present study investigated the viability, phagocytic capacity, and intracellular production
Antonella Cerino et al.
PloS one, 10(4), e0125704-e0125704 (2015-04-30)
We describe the production and characterization of human monoclonal antibodies (mAb) specific for the major hepatitis B virus (HBV) S protein. The mAbs, two IgG1κ and one IgG1λ, were secreted by B-cell clones obtained from peripheral blood mononuclear cells (PBMC)

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