推薦產品
品質等級
形狀
powder
技術
cell culture | mammalian: suitable
成分
L-glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
phenol red: yes
運輸包裝
ambient
儲存溫度
2-8°C
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一般說明
RPMI-1640培养基是在1966年由Moore及其同事在Roswell Park Memorial Institute开发的。该培养基通过对McCoy的5A培养基进行改良配制而成,用于在悬浮培养中支持淋巴母细胞生长,但后来经证还可支持各种不同贴壁依赖性细胞。RPMI 1640最初配合血清补充剂使用,但经证在无血清的情况下也能支持多种细胞系。它还广泛应用于杂交细胞融合方案和生长。该培养基适用于人正常白细胞和肿瘤白细胞=培养。
洛斯维帕克纪念研究所培养基通常被称为PRMI。 是一种用于细胞培养和组织培养的培养基。该培养基含有磷酸,适用于含有5%二氧化碳的气体。它可用于人淋巴细胞生长。
應用
RPMI-1640培养基用于以下应用:
- 用于细胞培养基(即在补充其它成分的 RPMI 1640 培养基中培养鼠胸腺瘤 EL-4 细胞)
- 用于脾细胞的分枝杆菌感染(在不含抗生素的 RPMI 1640 培养基中)
- 用于细胞分离和培养
- 用于淋巴细胞分离和培养、ELISPOT分析、体外增殖检测的培养基
- 用于gp39 基因表达和 CD40-免疫球蛋白结合检测的培养基
- 用于制备抗真菌剂
數量
按照每升培养基10.4克粉末配制。
重構
补充2.0 g/L碳酸氢钠。
推薦
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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文章
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
ELISpot 分析可提供免疫反應的定性與定量資訊,可顯示單一反應細胞的多種分泌產物。
條款
粉狀介質和鹽混合物具有極強的吸濕性,因此應防止大氣中的濕氣進入。
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.
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