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R6878

Sigma-Aldrich

Monoclonal Anti-phospho-Retinoblastoma (pSer795) antibody produced in mouse

~2 mg/mL, clone RB-10, purified immunoglobulin, buffered aqueous solution

同義詞:

Anti-phospho-Rb (pSer795)

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

RB-10, monoclonal

形狀

buffered aqueous solution

分子量

antigen 110 kDa

物種活性

mouse, rat, human, bovine, hamster

濃度

~2 mg/mL

技術

immunohistochemistry: suitable using 3% paraformaldehyde fixation, 0.15% Triton X-100 permeabilization
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361

同型

IgG1

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

phosphorylation (pSer795)

基因資訊

human ... RB1(5925)
mouse ... Rb1(19645)
rat ... Rb1(24708)

一般說明

Monoclonal Anti-phospho-Retinoblastoma (Rb) (pS795) (mouse IgG1 isotype) is derived from the RB-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic phospho peptide corresponding to the C-terminal amino acids of human retinoblastoma protein, conjugated to KLH. Retinoblastoma proteins p107, p110 (also designated Rb, or pRb) and p130, referred to collectively as pocket proteins, constitute a nuclear protein family that share a common structural unit (the pocket) dedicated to binding certain transcription factors and thereby regulating cellular proliferation. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with conserved LXCXE motif.

免疫原

synthetic phosphopeptide corresponding to the C-terminal amino acids 791-804 (pSer795) of human retinoblastoma protein conjugated to KLH.

應用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-phospho-Retinoblastoma (pSer795) antibody produced in mouse has been used in:
  • immunoblotting
  • immunocytochemistry
  • immunofluorescence
  • fluorescence-activated cell sorting (FACS) assays
  • enzyme linked immunosorbent assay (ELISA)

生化/生理作用

Retinoblastoma (Rb) exerts its function by repressing the transcription of cellular genes required for DNA replication and cell division. It can be phosphorylated at multiplicity of sites in vitro by cell division cycle protein 2 (Cdc2), cyclin-dependent kinase (Cdk2) and Cdk4/Cdk6 kinase complexes. Phosphorylation of pS795 is required to inactivate Rb imposed growth suppression.

外觀

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids


分析證明 (COA)

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The E2F1-3 transcription factors are essential for cellular proliferation
Wu L, et al.
Nature, 414(6862), 457-457 (2001)
Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells
Ohmine S, et al.
Oncology Reports, 39(4), 1725-1730 (2018)
Karen J Meaburn et al.
Aging cell, 6(2), 139-153 (2007-02-06)
A number of diseases associated with specific tissue degeneration and premature aging have mutations in the nuclear envelope proteins A-type lamins or emerin. Those diseases with A-type lamin mutation are inclusively termed laminopathies. Due to various hypothetical roles of nuclear
Cellular mechanisms of tumour suppression by the retinoblastoma gene
Burkhart DL and Sage J
Nature Reviews. Cancer, 8(9), 671-671 (2008)
Yan Li et al.
Molecular medicine reports, 14(3), 2222-2230 (2016-07-20)
Ginsenoside Rg3 (Rg3) is one of the primary constituents isolated from ginseng, and has been found to exhibit cytotoxic effects against cancer cells. The present study aimed to investigate the effects of Rg3 on human multiple myeloma cell proliferation and

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