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P3813

Sigma-Aldrich

Phosphate buffered saline

Phosphate buffered saline

powder, pH 7.4, for preparing 1 L solutions

同義詞:

PBS

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About This Item

MDL號碼:
MFCD00131855
分類程式碼代碼:
12161700
eCl@ss:
32129211
NACRES:
NA.25
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產品名稱

Phosphate buffered saline, powder, pH 7.4, for preparing 1 L solutions

品質等級

200

形狀

powder

儲存條件

dry at room temperature

顏色

white

pH值

7.4

溶解度

water: soluble

密度

1.19 g/cm3 at 20—25 °C
1.22 g/cm3 at 20—25 °C

應用

diagnostic assay manufacturing
life science and biopharma
sample preparation

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相關類別

一般說明

由于其多功能性和关键特性，磷酸盐缓冲液（PBS）是生物学研究中的基础试剂。其主要属性之一是等渗性，类似于人体的pH值、渗透压和离子浓度。这使PBS没有细胞毒性，令其在细胞生物学实验（如细胞清洗、组织运输以及细胞和试剂稀释）中起关键作用。这些应用确保了细胞活力和生理环境的保持。.

PBS的缓冲能力通过磷酸盐-磷酸平衡得到保持，它为实现最佳酶活力和各种生物学过程提供了至关重要的稳定pH 环境。这一特性使PBS成为试剂制备、蛋白质透析和免疫组织化学的理想选择，确保了一致稳定的实验结果。除了细胞生物学外，PBS也可用于多种领域，包括配制定制ELISA缓冲液，以确保最佳的抗体-抗原相互作用。此外，PBS可用作蛋白A/G 色谱的缓冲液，有助于基于特定抗原结合进行抗体纯化。在血液学和生物化学实验中，PBS被广泛用于清洗细胞和组织。

总之，PBS是生物学和生物化学研究中必不可少的工具，可提供等渗性、缓冲能力、简单性、稳定性。 细胞生物学、试剂制备、蛋白质纯化和各种生物学测试中的多样化应用突出了其在增进科学知识和理解中的重要性。

應用

它已被用于漂洗固定的组织，并为免疫定位过程创造合适的缓冲液环境（1） 它已被用作评估ELISA测试试剂盒性能的稀释剂（2）它已被用作免疫组织化学实验中的缓冲剂（3）。
磷酸盐缓冲液已用于：
  • 免疫荧光实验中的清洗溶液。[1]
  • 悬浮细菌细胞。[2]
  • 固定和免疫染色实验。[3]
  • 在免疫定位过程中清洗固定的组织，创造合适的缓冲液环境[4]
  • 作为评估ELISA测试试剂盒性能的稀释剂[5]
  • 作为免疫组织化学实验中的缓冲液 [6][3]

特點和優勢

  • 适用于各种研究应用的优质产品
  • 可用作生物学和生物化学应用的缓冲液成分
  • 易溶于水

包裝

锡箔袋包装

重構

当溶于1升蒸馏水或去离子水时，一小袋的物质将得到0.01 M磷酸盐缓冲盐水（NaCl 0.138 M；KCl - 0.0027 M)； pH 7.4, 25 °C时。

其他說明

如需了解生化试剂系列的更多信息，请填写此表。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000411453
0000411453
0000372018
0000372018
0000378719

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存取文件庫

Dynamic changes in lymphocyte GRK2 levels in cardiac transplant patients: a biomarker for left ventricular function.
Bonita RE et al.
Clinical and Translational Science, 3, 14-14 (2010)
Development of an internal control for evaluation and standardization of a quantitative PCR assay for detection of Helicobacter pylori in drinking water.
Sen K et al.
Applied and Environmental Microbiology, 73, 7380-7380 (2007)
Jennifer L Matthews et al.
Zebrafish, 15(3), 279-290 (2018-01-26)
Sperm cryopreservation is a highly efficient method for preserving genetic resources. It extends the reproductive period of males and significantly reduces costs normally associated with maintenance of live animal colonies. However, previous zebrafish (Danio rerio) cryopreservation methods have produced variable
Effect of the microtubule polymerizing agent taxol on contraction, Ca2+ transient and L-type Ca2+ current in rat ventricular myocytes.
Howarth FC et al.
The Journal of Physiology, 516, 409-409 (1999)
Anirudh Sharma et al.
Scientific reports, 8(1), 4916-4916 (2018-03-22)
Citrate-stabilized iron oxide magnetic nanoparticles (MNPs) were coated with one of carboxymethyl dextran (CM-dextran), polyethylene glycol-polyethylene imine (PEG-PEI), methoxy-PEG-phosphate+rutin, or dextran. They were characterized for size, zeta potential, hysteresis heating in an alternating magnetic field, dynamic magnetic susceptibility, and examined
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Questions

1–10 of 18 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Does P3813 PBS (Phosphate Buffered Saline) contain calcium or magnesium?

    1 answer

    1. The composition of P3813 PBS Phosphate Buffered Saline is considered proprietary, but the website provides the following information. When one pouch is dissolved in one liter of distilled or deionized water, it yields 0.01 M phosphate buffered saline with a pH of 7.4 at 25°C, consisting of NaCl at 0.138 M and KCl at 0.0027 M.

      Helpful?

  4. Is autoclaving necessary for PBS P3813 before conducting the presence-absence test for coliform bacteria?

    1 answer

    1. No, phosphate buffers by themselves are generally not autoclaved due to the common occurrence of precipitate formation. Autoclaving is commonly used in microbiology, but if your test method only requires sterilization without specifying autoclaving, filter sterilization might be a better option to consider.

      Helpful?

  5. Good morning, I would like to know what PKG means in kg. I would also like information about the validity of the solution resulting from the dissolution of the solid in question. Thanks

    1 answer

    1. PKG means package. One package is one pouch. As per its COA: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P3813-1PAK0000291289.pdf, each pouch contains 9.07-10.00 g of powder. And the pH and dissolution time are tested to validate the solution.

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  6. hi, what kg is in one package?

    1 answer

    1. The amount of powder to prepare 1 liter of buffer is approximately 9.55 grams.

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  7. Does this PBS contain Ca and Mg?

    1 answer

    1. No, the components of this formulation do not include calcium or magnesium.

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  8. What is the salinity of Phosphate buffer saline P3813?

    1 answer

    1. As mentioned on the product detail page, the contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.

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  9. How much is in each pouch of Product P3813, Phosphate buffered saline?

    1 answer

    1. Each pouch of phosphate buffered saline contains 8 g sodium chloride (e.g. Product No. S9625), 0.2 g potassium phosphate, monobasic (e.g. Product No. P5379), 1.15 g sodium phosphate, dibasic (e.g. Product No. S0876), 0.2 g potassium chloride (e.g. Product No. P4504), giving a total 9.55 g per packet.  This is the standard PBS formulation.

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  10. How long can solutions of Product P3813, Phosphate buffered saline, be kept once prepared?

    1 answer

    1. Sigma Aldrich has not evaluated the solution stability, but sterile solutions of PBS stored at 2-8°C can be expected to be stable for several months.

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