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重要文件

R4526

Sigma-Aldrich

定量PCR参考染料

100 ×, solution

同義詞:

qPCR参考染料, ROX

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About This Item

分類程式碼代碼:
41106300
NACRES:
NA.55
暫時無法取得訂價和供貨情況

形狀

solution

品質等級

用途

sufficient for ≥600 reactions

濃度

100 ×

技術

PCR: suitable

顏色

red

溶解度

water: soluble

儲存溫度

2-8°C

一般說明

Sigma′定量聚合酶链反应(qPCR)参考染料是一种专有染料,用于实时PCR。在使用SYBR绿、分子探针或双标记探针化学进行实时检测时,它可以用于反应数据的标准化。 我们提供100×参考染料,最大激发和放射峰分别出现在586nm和605nm。ROX参考染料仪器设定符合qPCR参考染料检测。

應用

定量 PCR 参考染料已被用于:
  • 制备用于实时定量聚合酶链反应 (RT-qPCR)的预混液[1][2]
  • 作为反应混合物的成分,用于通过定量聚合酶链式反应(qPCR)检测艰难梭菌[3][4]
  • 通过 qPCR 分析细胞 DNA 污染程度[5]

其他說明

定量PCR参考染料仅用于研发目的。不可用于药物、家庭或其他用途。

象形圖

Exclamation markEnvironment

訊號詞

Warning

危險分類

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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分析證明 (COA)

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Partial deletion of rng (RNase G)-enhanced homoethanol fermentation of xylose by the non-transgenic Escherichia coli RM10
Manow R, et al.
Journal of Industrial Microbiology & Biotechnology, 39(7), 977-985 (2012)
Araceli Melendez-Avalos et al.
Folia microbiologica, 65(1), 133-142 (2019-05-20)
This study aimed to analyze the proinflammatory cytokine mRNA expression in the urinary tract of BALB/c mice infected with bacterial strains with uropathogenic potential. Groups of four 6-week-old female BALB/c mice were intraurethrally inoculated with 5 × 107 colony-forming units (CFU) of
Gustav Johansson et al.
Molecular cancer therapeutics, 20(12), 2568-2576 (2021-09-24)
The majority of patients diagnosed with advanced gastrointestinal stromal tumors (GISTs) are successfully treated with a combination of surgery and tyrosine kinase inhibitors (TKIs). However, it remains challenging to monitor treatment efficacy and identify relapse early. Here, we utilized a
Increased environmental sample area and recovery of Clostridium difficile spores from hospital surfaces by quantitative PCR and enrichment culture
Brown KA, et al.
Infection Control and Hospital Epidemiology : The Official Journal of the Society of Hospital Epidemiologists of America, 39(8), 917-923 (2018)
Sambrook, J. et al.
Molecular Cloning: A Laboratory Manual, 3rd (2000)

文章

Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.

找出 SDS-PAGE 樣品製備挑戰的原因和補救方法,並優化電泳條件。

條款

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

我們的 SYBR Green qPCR 協定是專為檢測基因表達和 RT-PCR 反應的精確定量而設計的方法

相關內容

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

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我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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