推薦產品
用途
sufficient for 100 preparations
sufficient for 100 reactions
品質等級
特點
dNTPs included
hotstart
技術
qPCR: suitable
檢測方法
SYBR® Green
運輸包裝
wet ice
儲存溫度
−20°C
尋找類似的產品？ 前往 產品比較指南
一般說明
SYBR® Green Extract-N-Amp™ Plant PCR Kit is recommended for fast and reliable direct PCR from a wide range of plant leaf tissue.
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix™. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.
The SYBR Green Extract-N-Amp Plant PCR Kit contains all the reagents needed for rapid extraction of genomic DNA from plant leaf tissue coupled with direct polymerase chain reaction (PCR) amplification and real-time detection. The need for freezing plant tissue in liquid nitrogen or mechanical disruption and complete genomic DNA purification has been eliminated.
Two solutions provided in the kit combine to release genomic DNA from leaf tissue and neutralize substances inhibitory to PCR. The genomic DNA released into solution serves as a template for the SYBR Green Extract-N-Amp PCR ReadyMix™. This ReadyMix, which contains SYBR Green fluorescent dye, Taq, JumpStart antibody, dNTPs and MgCl2, is specially formulated to amplify target sequences in the cellular lysate.
特點和優勢
- Single-step extraction of plant genomic DNA for polymerase chain reaction (PCR) in less than 15 minutes
- No freezing, mechanical disruption, organic extraction, column purification, or precipitation required
- Specially formulated PCR ReadyMix for use with extract
- Hot Start antibody for highly specific PCR amplification of genomic DNA
- No loading buffers or tracking dyes required for gel analysis
- SYBR® Green I dye acts as a nonspecific reporter for real-time PCR
- SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
- Compatible with high-throughput requirements for genetic analysis of plants
- No long enzymatic digestions
- Extract stable at 4°C for at least 6 months
其他說明
该实验方案采用20 μl规格的PCR反应。
法律資訊
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
相關產品
產品號碼
描述
訂價
訊號詞
Warning
危險分類
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
客戶也查看了
Trenis D Palmer et al.
Journal of visualized experiments : JoVE, (51)(51), doi:10-doi:10 (2011-06-16)
During metastasis cancer cells disseminate from the primary tumor, invade into surrounding tissues, and spread to distant organs. Metastasis is a complex process that can involve many tissue types, span variable time periods, and often occur deep within organs, making
文章
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
簡單的 DNA/RNA 純化方法有助於從各種來源進行基因組分析，提高研究效率。
Active Filters
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務
