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重要文件

M8787

Sigma-Aldrich

氯化镁 溶液

PCR Reagent, 25 mM MgCI2 solution for PCR

同義詞:

PCR optimization

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About This Item

線性公式:
MgCl2
CAS號碼:
分子量::
95.21
MDL號碼:
分類程式碼代碼:
12352302
PubChem物質ID:
NACRES:
NA.52
暫時無法取得訂價和供貨情況

品質等級

200
300

形狀

liquid

包裝

vial of 1.5 mL

濃度

25 mM±1 mM

技術

PCR: suitable

顏色

colorless

應用

agriculture
diagnostic assay manufacturing

異物活動

DNase, RNase, none detected

儲存溫度

2-8°C

SMILES 字串

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI 密鑰

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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一般說明

氯化镁溶液适用于聚合酶链式反应(PCR)优化。氯化镁溶液提供聚合酶链式反应(PCR)所需镁离子。影响引物-模板退火温度、保真度、特异性和产量。过量镁会导致非特异性产物增加,镁缺乏会导致产量降低。

應用

氯化镁溶液已用作以下材料的组分:

  • 聚合酶链式反应(PCR)扩增混合液[1]
  • 简单序列重复(ISSR)-PCR扩增混合液[2]
  • 16S-23S rRNA基因内转录间隔区(ITS)PCR扩增及限制性内切分析(ITS-PCR- rflp)反应缓冲液[3]
  • 用于定量逆转录-聚合酶链反应(qRT PCR)的PCR反应混合物,以扩增逆转录cDNA[4]
  • 用于RT-PCR检测基因表达的反应混合物[5]

適合性

适用于聚合酶链反应的优化。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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分析證明 (COA)

Lot/Batch Number

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存取文件庫

Molecular characterization of Fusarium oxysporum f. sp. cubense isolates from banana
Das A, et al.
Pest Management in Horticultural Ecosystems , 18(2), 171-178 (2012)
Massimiliano Bergallo et al.
American journal of perinatology, 36(10), 1060-1065 (2018-12-01)
Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and
Genome subtyping of autochthonous Bacillus species isolated from Iru, a fermented Parkia biglobosa seed
Adewumi G A, et al.
Food Biotechnology, 28(3), 250-268 (2014)
Kadri Õunap et al.
PloS one, 10(7), e0133841-e0133841 (2015-07-28)
The human WBSCR22 protein is a 18S rRNA methyltransferase involved in pre-rRNA processing and ribosome 40S subunit biogenesis. Recent studies have shown that the protein function in ribosome synthesis is independent of its enzymatic activity. In this work, we have
Takeshi Tomita et al.
PloS one, 9(9), e108957-e108957 (2014-10-01)
Celastramycin A, a small molecule that inhibits the production of antibacterial peptides in an ex vivo culture system of Drosophila, suppresses the TNFα-mediated induction of IL-8 in mammalian cells. To understand its molecular mechanism, we examined Celastramycin A binding proteins

文章

Quality DNA crucial for meaningful data; intact DNA key for successful chromosomal aberration analysis from FFPE tissues.

SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.

條款

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions

我們的 SYBR Green qPCR 協定是專為檢測基因表達和 RT-PCR 反應的精確定量而設計的方法

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KOD One™ PCR 主混合物概述,用於高特異性、高保真度和高產量的超快速 PCR

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

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