推薦產品
產品名稱
邻苯二胺 二盐酸盐, tablet, 4 mg substrate per tablet
形狀
tablet
mp
258 °C (dec.) (lit.)
儲存溫度
2-8°C
SMILES 字串
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI 密鑰
RIIWUGSYXOBDMC-UHFFFAOYSA-N
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一般說明
邻苯二胺二盐酸盐（OPD）是一种显色底物，因为利用辣根过氧化物酶缀合物，因此可用于酶联免疫吸附试验（ELISA）程序。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。
應用
在酶联免疫吸附试验（ELISA）中，邻苯二胺盐酸盐用作辣根过氧化物酶偶联二抗的底物。
重構
将 1 片片剂溶于 pH 值为 5.0的0.05M磷酸盐 - 柠檬酸盐缓冲液中至所需浓度（通常使用0.4mg / ml的OPD浓度）。临用前，向 100 mL 底物缓冲液中加入 40μl 新鲜过氧化氢（浓度为 30%）。片剂单独包装在铝箔包装中。
訊號詞
Warning
危險分類
Aquatic Chronic 2 - Carc. 2 - Muta. 2 - Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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文章
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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