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重要文件

P8287

Sigma-Aldrich

邻苯二胺 二盐酸盐

chromogenic, tablet

同義詞:

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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About This Item

線性公式:
C6H4(NH2)2·2HCl
CAS號碼:
分子量::
181.06
Beilstein:
3912045
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83
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產品名稱

邻苯二胺 二盐酸盐, tablet, 10 mg substrate per tablet

品質等級

形狀

tablet

mp

258 °C (dec.) (lit.)

儲存溫度

2-8°C

SMILES 字串

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI 密鑰

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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相關類別

一般說明

邻苯二胺(o-PD)是一种无色的非荧光分子。[1]
邻苯二胺二盐酸盐(OPD)是一种显色底物,因为利用辣根过氧化物酶缀合物,因此可用于酶联免疫吸附试验(ELISA)程序。底物产生可溶的最终产物,其颜色为橙棕色,并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应,并在492 nm处读取。

應用

在酶联免疫吸附试验(ELISA)中,邻苯二胺盐酸盐用作辣根过氧化物酶偶联二抗的底物。[2][3][4]

重構

将一片药片溶于 pH 5.0 的 0.05M 磷酸盐-柠檬酸盐缓冲液中至所需浓度(通常使用0.4mg/ml 的 OPD 浓度)。使用前,在每 100ml 底物缓冲溶液中加入 40 μl 新鲜的 30% 过氧化氢,现配现用。片剂分别包装在箔包内。

訊號詞

Warning

危險分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1


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Sara Buezo Montero et al.
Frontiers in cellular and infection microbiology, 10, 377-377 (2020-08-28)
Mosquitoes of the Aedes genus transmit arboviruses of great importance to human health as dengue, chikungunya, Zika and yellow fever. The tiger mosquito Aedes albopictus can play an important role as arboviral vector, especially when Aedes aegypti is absent or
Henry Surendra et al.
Malaria journal, 18(1), 227-227 (2019-07-10)
Analysis of anti-malarial antibody responses has the potential to improve characterization of the variation in exposure to infection in low transmission settings, where conventional measures, such as entomological estimates and parasitaemia point prevalence become less sensitive and expensive to measure.
Rama Devudu Puligedda et al.
Antibodies (Basel, Switzerland), 9(1) (2020-03-04)
Poliovirus (PV)-specific intestinal IgAs are important for cessation of PV shedding in the gastrointestinal tract following an acute infection with wild type or vaccine-derived PV strains. We sought to produce IgA monoclonal antibodies (mAbs) with PV neutralizing activity. We first
Ted M Ross et al.
Vaccine, 37(17), 2369-2376 (2019-03-25)
Since the first identification of the H5N1 Goose/Guangdong lineage in 1996, this highly pathogenic avian influenza virus has spread worldwide, becoming endemic in domestic poultry. Sporadic transmission to humans has raised concerns of a potential pandemic and underscores the need
Saima Ajaz et al.
American journal of physiology. Cell physiology, 320(1), C57-C65 (2020-11-06)
The COVID-19 pandemic has been the primary global health issue since its outbreak in December 2019. Patients with metabolic syndrome suffer from severe complications and a higher mortality rate due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We

文章

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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