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重要文件

P3910

Sigma-Aldrich

蛋白酶 来源于地衣芽孢杆菌

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

同義詞:

Subtilisin A, Subtilisin Carslberg, 枯草杆菌蛋白酶A, 蛋白酶 来源于地衣芽孢杆菌

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.84
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形狀

lyophilized powder

品質

for use in Total Dietary Fiber Assay, TDF-100A

分子量

27 kDa

應用

food and beverages

運輸包裝

wet ice

儲存溫度

2-8°C

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相關類別

特異性

枯草杆菌蛋白酶A是丝氨酸S8内蛋白酶家族的一员。它具有宽泛的特异性并在P1位对大的未带点残基具有偏好性。它可水解天然和变性的蛋白,并在碱性条件下具有活性。

應用

这是从地衣芽孢杆菌发酵中分离得到的蛋白水解酶。它是一种对天然和变性蛋白具有广泛特异性的丝氨酸内切蛋白酶,并在碱性条件下具有活性。它用于总膳食纤维检测(TDF-100A)中。

生化/生理作用

蛋白酶可通过肽键的水解使蛋白质分解代谢。 蛋白酶可被丝氨酸活性位点抑制剂所灭活,如苯甲基磺酰氟(PMSF)和二异丙基氟磷酸盐。

物理性質

枯草杆菌蛋白酶是一种具有27 KDa分子量并不含有二硫键的非糖基化单一多肽链。

單位定義

在pH 7.5、37°C条件下,一个单位每分钟可水解酪蛋白,生成相当于1.0 μmole (181 μg)酪氨酸的颜色。

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Questions

  1. 본 제품에 대한 U/mg 값을 알고싶습니다.

    1 answer
    1. This product is intended for use as a component for the Total Dietary Fiber Assay kit, TDF-100A. Unfortunately, the activity is not determined. See the link below to review Protease from Bacillus licheniformis products that are tested for activity:
      https://www.sigmaaldrich.com/substance/proteasefrombacilluslicheniformis123459014011

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