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一般說明
该酶在pH 8-10下稳定,可在24小时保存高达90%的活性。其55-60℃下具有最大活性。
特異性
枯草素A 是丝氨酸S8内蛋白酶家族的一个成员。它具有广泛的特异性,在P1位置有较大的非荷电残基。它可水解天然和变性蛋白,并且在碱性条件下具有活性。
應用
该产品已与其他酶一起用于 原位 蛋白水解,以制备适用于结构测定的晶体。它还被用于分离可用于 体外 功能研究的肌膜下(SS)和肌原纤维间(IMF)线粒体。
这是由 衣芽孢杆菌发酵而分离出来的一种蛋白水解酶。这是一种丝氨酸内蛋白酶,对天然和变性蛋白具有广泛的特异性,在碱性条件下具有活性。
生化/生理作用
蛋白酶通过水解肽键分解蛋白质。 蛋白酶可被丝氨酸活性位点抑制剂灭活,如苯基甲基磺酰氟(PMSF)和二异丙基氟磷酸酯。 蛋白酶,P5380的产物,是一种丝氨酸内蛋白酶,对天然和变性蛋白具有广泛的特异性,在碱性条件下具有活性。 它在某些有机溶剂中具有活性,如干辛烷。
物理性質
枯草素 是一种无二硫键的非糖基化单肽链,其分子量为27kda。
單位定義
一单位在37°C、pH 7.5条件下每分钟可水解1.0μmole(181 μg)的酪氨酸(福林酚试剂显色)。
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
從最近期的版本中選擇一個:
分析證明 (COA)
Lot/Batch Number
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Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
使用本方案,可在石蠟包埋組織切片中透過染色和顯現特定抗原,進行整個免疫組織化學 (IHC) 程序。
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