The concentration of BSE in this product is approximately 100 mg/mL in DPBS. The lot-specific concentration is reported in the Certificate of Analysis as "Protein by Biuret". Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/o3008#product-documentation
推薦產品
生物源
bovine
無菌
sterile-filtered
產品線
BioReagent
形狀
liquid
成份
Oleic acid, 2 mol/mol albumin
濃度
100 mg/mL BSA in DPBS
90-120 mg/mL protein (biuret)
技術
cell culture | mammalian: suitable
雜質
endotoxin, tested
儲存溫度
2-8°C
相關類別
相關產品
產品號碼
描述
訂價
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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文章
Supplement cell culture systems with fatty acids for biomanufacturing heterologous proteins like monoclonal antibodies.
不飽和脂肪酸、油酸和其他細胞培養成分如何影響用於生物製造異源蛋白質（包括單克隆抗體）的無血清、無蛋白質細胞培養系統的性能。
How the unsaturated fatty acid, oleic acid and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.
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What is the bsa concentration in the solution?
1 answer-
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Is it suitable to store this reagent in -20C in aliquots? We received our order recently and the shipping box has a large label of "Do not Freeze" I was inquiring if there was a decrease in concentration if frozen?
1 answer-
To effectively preserve the integrity of this product, it should be stored at the recommended temperature and protected from light. The stability and optimal performance may be impacted by the freeze/thaw process. This product should be stored at 2 to 8 C.
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How is the binding of oleic acid to BSA is confirmed?
1 answer-
Many fatty acids have a natural affinity to some plasma proteins. In this case BSA serves as a carrier or transporter of the insoluble oleic acid. The product is tested for protein and oleic acid content, only. No additional testing is performed to confirm the complex formation. See the following publications for further information:
https://pubmed.ncbi.nlm.nih.gov/236351/
https://pubmed.ncbi.nlm.nih.gov/19745557/#:~:text=Albumin%20acts%20as%20main%20fatty,a%20several%20orders%20of%20magnitudeHelpful?
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What is the concentration of Oleic Acid in this product in Molarity (M) or milimolarity (mM)? I need this to be able to follow a protocol
1 answer-
Oleic acid has a molecular mass of 282.47 g/mol. In order to find the concentration of oleic acid in a specific lot, use the Oleic acid concentration on the lot-specific certificate of analysis (COA).
The equation used to find the concentration is as follows: (molar mass of Oleic acid) x (concentration of Oleic acid from COA), this will result in units of liter/mole which can be multiplied by the reciprocal, resulting in units of Molarity (mole/liter).
For example, for lot number SLCM9550, the Oleic acid concentration is reported as 940 ug/mL.
282.47 g/mol / 0.94 g/L =300.5 L/mol
1/300.5= 0.003327 M (mol/liter) = 3.33 mM Oleic AcidHelpful?
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Through what method is oleic acid complexed to BSA?
1 answer-
Unfortunately, the method of preparation considered proprietary.
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