推薦產品
用途
sufficient for 4 purifications
環保替代產品特色
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
技術
DNA purification: suitable
環保替代類別
儲存溫度
15-25°C
一般說明
GenElute HP质粒大量制备试剂盒可以简单、快速、低成本的从重组大肠杆菌培养物中分离质粒DNA。该试剂盒采用了一种可快速清除裂解液的过滤针筒和一种适于真空或离心形式的硅胶结合柱。从150 mL Luria Broth (LB)培养基过夜培养物中，可分离出多达1.2 mg 的质粒DNA。请注意，实际得率取决于所用的菌株、质粒和培养基。
通过离心分离法收集过夜培养的重组大肠杆菌培养物，并通过改性SDS碱裂解法进行处理，之后再在高盐环境下使DNA吸附到硅胶膜上。通过两遍洗涤步骤，去除污染物。最后，在洗脱溶液(Tris-HCl)或水中洗脱结合所得的DNA。
回收的质粒DNA主要以超螺旋形式存在。此类DNA可直接供限制性酶切、连接、测序、PCR、转化和转染等下游应用使用。
通过离心分离法收集过夜培养的重组大肠杆菌培养物，并通过改性SDS碱裂解法进行处理，之后再在高盐环境下使DNA吸附到硅胶膜上。通过两遍洗涤步骤，去除污染物。最后，在洗脱溶液(Tris-HCl)或水中洗脱结合所得的DNA。
回收的质粒DNA主要以超螺旋形式存在。此类DNA可直接供限制性酶切、连接、测序、PCR、转化和转染等下游应用使用。
Sigma Life Science致力于为您提供符合一项或多项绿色化学十二条原则 （The 12 Principles of Greener Chemistry），更环保的替代产品。相对于使用苯酚和氯仿进行DNA提取的标准方法，本产品符合固有安全化学原则(Inherently Safer Chemistry)。
應用
GenElute™ HP Plasmid Maxiprep Kit has been used to isolate plasmid DNA from Escherichia coli.[1]
特點和優勢
- 可在30分钟内从收集所得的细菌培养物中纯化出质粒DNA
- 可纯化出多达1.2 mg的高拷贝数质粒DNA
- 真空或离心形式可灵活选择
- 相对于其他快速试剂盒，所含的塑料成分更少，减少了废物量
- 无需进行苯酚/氯仿萃取或乙醇沉淀步骤
其他說明
更多信息，请见www.sigma-aldrich.com/genelutehp。
法律資訊
GenElute is a trademark of Sigma-Aldrich Co. LLC
訊號詞
Danger
危險分類
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
標靶器官
Central nervous system
儲存類別代碼
3 - Flammable liquids
閃點（°F）
77.0 °F - closed cup
閃點（°C）
25 °C - closed cup
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條款
Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.
相關內容
Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.
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