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NA0160

Sigma-Aldrich

GenElute HP 质粒小量制备试剂盒

greener alternative

sufficient for 350 preparations

同義詞:

GenElute HP质粒试剂盒, Gen Elute

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About This Item

分類程式碼代碼:
41105501
NACRES:
NA.52

用途

sufficient for 350 preparations

環保替代產品特色

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技術

DNA extraction: suitable

環保替代類別

儲存溫度

15-25°C

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一般說明

通过离心收集过夜培养的重组 大肠杆菌,并进行改良的碱-SDS裂解步骤,然后在高盐存在下将质粒DNA吸附至二氧化硅上。然后,通过真空或旋转洗涤步骤去除污染物。最后,用水或Tris-EDTA缓冲液对结合的质粒DNA进行洗脱。

GenElute HP质粒试剂盒为从 大肠杆菌 培养物中进行质粒制备提供了一种超快速有效的解决方案。该试剂盒结合了基于二氧化硅的膜技术和旋转或真空形式的便利性,可在不到30分钟的时间内从1-5 mL的 大肠杆菌 培养物中最高可达获得25 μg的高拷贝质粒DNA。注意,实际的产量和最佳培养量取决于质粒和培养基。
我们致力于为您带来符合一项或多项绿色化学12原则的环保替代产品。相比标准DNA提取方法使用苯酚和氯仿,该产品是一种更安全的化学产品。

應用

分离所得的质粒DNA可直接用于限制性酶切、克隆、PCR、转录和测序等下游应用。

特點和優勢

  • 可在30分钟内获得纯化的质粒DNA
  • 可纯化出多达25 μg高拷贝数质粒DNA
  • 真空或离心形式可灵活选择
  • 无需进行苯酚/氯仿萃取或乙醇沉淀步骤

法律資訊

GenElute is a trademark of Sigma-Aldrich Co. LLC

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

標靶器官

Central nervous system

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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