The kit is intended for the isolation of intact mitochondria, so most of the isolated mitochondria will contain intact inner and outer membranes. While trace amounts of mitochondria-associated membranes (MAMs) may co-purify during the process, the protocol is not optimized to enrich or preserve MAMs. Please see the Product Information Sheet at the link below for further details.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/204/819/mitoiso1pis-ms.pdf
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品質等級
用途
sufficient for 10-20 g (animal tissue)
sufficient for 50 assays (2 mL)
儲存期限
2 yr
技術
fractionation: suitable
運輸包裝
dry ice
儲存溫度
−20°C
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應用
线粒体分离试剂盒可简单、快速地分离动物组织中富集的线粒体组分，并检测线粒体内膜的电化学质子梯度(ΔΨ)。用于线粒体介导的细胞凋亡研究。
特點和優勢
- 特制提取剂和可靠实验方案适合少量分离——在离心管中即可分离富集完整线粒体组分。
- 获得完整有活性的线粒体——所得线粒体适用于细胞凋亡、氧化应激等体外分析研究。
- 提供可确定内膜完整性的染色剂和实验方案——无需购买其他试剂，即可确定线粒体内膜完整性。
- 适配细胞色素C氧化酶检测试剂盒——可轻松确定线粒体外膜完整性。
相關產品
產品號碼
描述
訂價
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
10 - Combustible liquids
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
使用 Annexin V、Caspase 和 TUNEL DNA 片段檢測法進行細胞凋亡檢測。
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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Is it possible to use the MITOISO1 kit to also isolate MAMs?
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Where is the step to get cytosol?
1 answer-
The kit is intended for the isolation of intact mitochondria. It was not developed for cytoplasma collection. Nevertheless, reviewing the protocol, it can be assumed that the cytoplasmic fraction should be in the supernatant after the second centrifugation (point 8 of the Preparation of mitochondria from soft tissues protocol). Please keep in mind that this is not tested for suitability for cytoplasmic fraction.
Please see product MIT1000 Mitochondria/Cytosol Fractionation Kit for efficient isolation of intact mitochondria from cultured cells. This kit can be used for tissue samples as long as the tissue is first homogenized into a single cell suspension. It may be helpful to use the same protocol that is recommended for cells following homogenization of the tissue into a single cell suspension in the supplied Isotonic Mitochondrial Buffer containing protease inhibitors, then follow the kits protocol for cells: https://www.sigmaaldrich.com/product/mm/mit1000.
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Are the extraction buffers from Product MITOISO1, Mitochondria Isolation Kit, available separately?
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The extraction buffers supplied with this kit are only available with the kit.
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When using Product MITOISO2, Mitochondria Isolation Kit, how can I store my isolated mitochondria?
1 answer-
Please see below for information on how best to store your isolated mitochondria using the MITOISO2 kit:If only interested in the proteins isolated from mitochondria, storage at -20 deg C is recommended. If intact mitochondria are required, storage at 4 deg C is recommended. These intact mitochondria are stable after refrigeration for up to 24 hours.
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When using Product MITOISO1, Mitochondria Isolation Kit, how long can I store my isolated mitochondria?
1 answer-
If only interested in the proteins isolated from mitochondria, storage at -20 deg C is recommended. If intact mitochondria are required, storage at 4 deg C is recommended. These intact mitochondria are stable after refrigeration for up to 24 hours.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How much tissue can I use with Product MITOISO1, Mitochondria Isolation Kit,?
1 answer-
The procedures described in the bulletin are for small amounts of tissue samples (50-200 mg). Larger amounts of tissue can be purified with modifications listed in the technical bulletin. We recommend a minimum of 50 mg.
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Is the Product MITOISO1, Mitochondria Isolation Kit, JC-1 stain available separately?
1 answer-
Yes, while the component J4519 is not available separately, JC-1 stain is listed separately as Sigma Product No. T4069 or Product No. 40882.
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Can Product MITOISO1, Mitochondria Isolation Kit, be used for isolation of mitochondria from cultured cells?
1 answer-
It is possible to use this kit for cultured cells, however MITOISO2 is a kit that was specifically designed for this purpose. This kit was originally designed for isolation from tissues.
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