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CYTOCOX1

Sigma-Aldrich

细胞色素c氧化酶检测试剂盒

sufficient for 100 tests, soluble and membrane bound mitochondria

同義詞:

细胞色素C氧化酶活性检测试剂盒

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84
暫時無法取得訂價和供貨情況

品質等級

200

用途

sufficient for 100 tests

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

human ... COX1(4512)

相關類別

應用

细胞色素c氧化酶检测试剂盒使用了一种经过优化的比色检测方法，该方法基于550 nm处测得的高铁细胞色素c吸光度下降（这是由于高铁细胞色素c被细胞色素c氧化酶氧化造成的）进行检测。[1] 该试剂盒适于检测线粒体外膜完整度/线粒体应激性，同时也适于检测亚细胞组分之中的线粒体。[2]

生化／生理作用

细胞色素c氧化酶[EC 1.9.3.1]位于线粒体内膜上,将线粒体基质和膜间隙分离开来，一直被用作这种膜的标记物。在细菌的细胞质膜上，也存在细胞色素c氧化酶。细胞色素c氧化酶可通过氧化磷酸化过程连接细胞色素链之间的电子转运，为细胞提供能量。

特點和優勢

  • 简单，经优化的实验方案 - 无需进行专门的培训，即可获得可重复的结果
  • 可用于测定任意线粒体来源的细胞色素酶c的活性 - 这种酶在所有种类生物的线粒体中均存在
  • 可用于检测亚细胞成分中是否存在线粒体 - 节省时间，同时提高了细胞器制备质量的可信度
  • 可结合MITOISO1 Mitochondrial Isolation Kit使用- 标准化的线粒体制备和分析，确保了可重复性
  • 可用于分析线粒体膜的完整性
  • 简单的比色测定解决方案

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • C2506Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mgSDS
  • D4641n-Dodecyl β-D-maltoside, ≥98% (GC) 10 mgSDS

象形圖

Corrosion
GHS05

訊號詞

Danger

危險聲明

H315,H318

危險分類

Eye Dam. 1 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000306825
0000366354
0000366354
0000306825
0000184898

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Filip J Larsen et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 25(8), 2843-2852 (2011-05-18)
The basal metabolic rate (BMR) is referred to as the minimal rate of metabolism required to support basic body functions. It is well known that individual BMR varies greatly, even when correcting for body weight, fat content, and thyroid hormone
Hikaru Miyagi et al.
The Journal of biological chemistry, 284(12), 7553-7560 (2009-01-23)
Cells of the yeast Saccharomyces cerevisiae contain three NAD kinases; namely, cytosolic Utr1p, cytosolic Yef1p, and mitochondrial Pos5p. Previously, the NADH kinase reaction catalyzed by Pos5p, rather than the NAD kinase reaction followed by the NADP(+)-dependent dehydrogenase reaction, had been
Chao-Chin Liu et al.
Frontiers in microbiology, 11, 170-170 (2020-03-03)
Staphylococcus aureus spreads rapidly on the surface of soft agar medium. The spreading depends on the synthesis of biosurfactants, i.e., phenol soluble modulins (PSMs), which facilitate colony spreading of S. aureus. Our earlier study demonstrated that water accumulates in a
Chiori Omori et al.
Nutritional neuroscience, 20(9), 538-546 (2016-06-23)
The activities of mitochondrial enzymes, which are essential for neural function, decline with age and in age-related disease. In particular, the activity of cytochrome c oxidase (COX/complex IV) decreases in patients with Alzheimer's disease (AD). COX, a mitochondrial inner membrane
Filip J Larsen et al.
Cell metabolism, 13(2), 149-159 (2011-02-03)
Nitrate, an inorganic anion abundant in vegetables, is converted in vivo to bioactive nitrogen oxides including NO. We recently demonstrated that dietary nitrate reduces oxygen cost during physical exercise, but the mechanism remains unknown. In a double-blind crossover trial we
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文章

细胞器分离

亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

細胞凋亡測試

使用 Annexin V、Caspase 和 TUNEL DNA 片段檢測法進行細胞凋亡檢測。

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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