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D8938

Sigma-Aldrich

KIMBLE Dounce组织研磨套装

KIMBLE Dounce tissue grinder set

2 mL complete

同義詞:

885300-0002, Dounce, Dounce Homogenizer, Glass Tissue Grinder, KIMBLE Tissue Grinder, KONTES, Pestles, Tissue Grinder, Tubes

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About This Item

分類程式碼代碼:
41121800
NACRES:
NB.22
暫時無法取得訂價和供貨情況

材料

glass

特點

autoclavable

製造商/商標名

Kimble® 885300-0002

杵 A 間隙

0.0030-0.0050 in.

杵 B 間隙

0.0005-0.0025 in.

吸頭容量 × 濃縮體積

2 mL × 60 mm

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一般說明

主要为细胞工作设计,在匀浆后细胞核保持完整。全玻璃结构。每一套配有两根研杵。大间隙研杵用于初始样本降解。小间隙研杵用于最终匀浆。

特點和優勢

• Manufactured from borosilicate glass 3.3• Designed primarily for cellular work where the nucleus remains intact after homogenization.• All-glass construction• Two pestles are supplied with each complete unit• Large clearance pestle is used for the initial sample reduction• Small clearance pestle is used to form the final homogenate• Replacement components are available and completely interchangeable

法律資訊

KIMBLE is a registered trademark of DWK Life Sciences

從最近期的版本中選擇一個:

分析證明 (COA)

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Fang-Yuan Hu et al.
Frontiers in bioengineering and biotechnology, 8, 564057-564057 (2020-10-20)
Retina is a crucial tissue for capturing and processing light stimulus. It is critical to describe the characteristics of retina at the single-cell level for understanding its biological functions. A variety of abnormalities in terms of morphology and function are
Naomi Habib et al.
Nature neuroscience, 23(6), 701-706 (2020-04-29)
The role of non-neuronal cells in Alzheimer's disease progression has not been fully elucidated. Using single-nucleus RNA sequencing, we identified a population of disease-associated astrocytes in an Alzheimer's disease mouse model. These disease-associated astrocytes appeared at early disease stages and
Naomi Habib et al.
Nature methods, 14(10), 955-958 (2017-08-29)
Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human
Benjamin C Reiner et al.
G3 (Bethesda, Md.), 10(5), 1647-1655 (2020-03-07)
Long interspersed element-1 retrotransposons (LINE-1 or L1) are ∼6 kb mobile DNA elements implicated in the origins of many Mendelian and complex diseases. The actively retrotransposing L1s are mostly limited to the L1 human specific (L1Hs) transcriptional active (Ta) subfamily.
Ana J Chucair-Elliott et al.
Communications biology, 3(1), 693-693 (2020-11-21)
Epigenetic regulation of gene expression occurs in a cell type-specific manner. Current cell-type specific neuroepigenetic studies rely on cell sorting methods that can alter cell phenotype and introduce potential confounds. Here we demonstrate and validate a Nuclear Tagging and Translating

Questions

1–2 of 2 Questions  
  1. Is it possible to purchase individual pestles for the D8938 KIMBLE Dounce tissue grinder set?

    Is it possible to purchase individual pestles for the D8938 KIMBLE Dounce tissue grinder set?

    1 answer
    1. This product is only sold as a set. You may want to check with the manufacturer to inquire if the pestles can be obtained separately. For further information, you can contact them at the following link: https://www.dwk.com/na/contact-us. The Kimble catalog number for this item is Kimble® 885300-0002.

      Helpful?

  2. Can we use this double homogeniser for mitochondria isolation from cells?

    1 answer
    1. This product has not been validated for use in mitochondrial isolation. This is an application that will need to be determined by the end-user, however there are a number of publications citing this product in organelle isolation. It may be helpful to review page 12 of the following journal article:
      https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/febs.14028

      It also may be helpful to view the peer-reviewed articles:
      https://www.sigmaaldrich.com/search/d8938?focus=papers&page=1&perpage=30&sort=relevance&term=d8938&type=citation_search

      Helpful?

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