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MAK359

Sigma-Aldrich

线粒体复合体I活性检测试剂盒

sufficient for 100 colorimetric tests

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About This Item

分類程式碼代碼:
12352200
暫時無法取得訂價和供貨情況

用途

sufficient for 100 colorimetric tests

檢測方法

colorimetric

一般說明

线粒体复合物I或NADH:泛醌氧化还原酶是位于线粒体膜上的电子传输链的第一个也是最大的复合物。它将NADH氧化为NAD+，并将电子转移至泛醌（也存在于线粒体内膜中），将其转化为泛醇。在这个过程中，它通过线粒体内膜运输质子，有助于形成电化学梯度。该过程对于细胞呼吸非常重要，对复合物I活性的不利影响会损害线粒体呼吸，从而进一步导致细胞应激。

適合性

适用于测量分离的线粒体中的线粒体复合物I活性。

原則

线粒体复合物I测定试剂盒是一种快速可靠的测定分离线粒体中复合物I活性的方法。它可用于分离的线粒体中的呼吸研究，并可用于研究毒物，药物和其他环境条件对线粒体复合物I活性的影响。该试剂盒使用癸基泛醌（泛醌的类似物）作为电子受体，该电子受体通过络合物I的催化活性转化为癸基泛醇。络合物I染料以氧化形式吸收A600 nm处的光，用作末端电子受体。接受来自癸基泛醇的电子。通过记录还原的复合物I染料在A600 nm处的吸光度变化，通过比色法确定复合物I的活性。通过从总活性中减去在复合物I抑制剂鱼藤酮存在下的活性来获得特定的复合物I活性。该试剂盒可检测低至0.1 mU/孔，线性高达7 mU/孔。

危險聲明

H412

防範說明

P273 - P501

危險分類

Aquatic Chronic 3

儲存類別代碼

10 - Combustible liquids

閃點（°F）

188.6 °F

閃點（°C）

87 °C

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分析證明 (COA)

Lot/Batch Number
8H24K09680

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Ahmed Ismaeel et al.
Nutrients, 14(5) (2022-03-11)
Previous studies have shown that chronic heavy alcohol consumption and consumption of a high-fat (HF) diet can independently contribute to skeletal muscle oxidative stress and mitochondrial dysfunction, yet the concurrent effect of these risk factors remains unclear. We aimed to

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  1. How is shipping temperature determined? And how is it related to the product storage temperature?

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    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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