Here is the recommended protocol for cell sample preparation:
1. Homogenize cells with the heme reagent which contains NaOH and detergent which will effectively lyse the cells.
2. Let incubate for 5 min for the color to develop, then centrifuge to pellet any debris.
3. Transfer 250 µL of clear supernatant to a 96 well plate and measure the absorbance at 400 nm.
推薦產品
檢測方法
colorimetric
相關疾病
hematological disorder
儲存溫度
2-8°C
一般說明
特點和優勢
与高通量处理系统兼容。
適合性
适用于血液、血浆、血清、尿液、携带酶的血红素等多种生物样品中血红素的定量测定及药物对血红素代谢影响的评价。
原則
血红素含量测定基于碱性水溶液法，其中血红素转化为均匀的有色形式，产生比色 (400 nm) 结果，与样品中的血红素浓度成正比。优化后的配方减少了干扰，表现出较高的灵敏度。1 mg/dL 血红素等于 15.3M、0.001% 或 10ppm。该试剂盒 96 孔格式的线性检测范围为 0.6-125M 血红素。
訊號詞
Warning
危險聲明
危險分類
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1
儲存類別代碼
8B - Non-combustible corrosive hazardous materials
閃點（°F）
Not applicable
閃點（°C）
Not applicable
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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How do you use this product on cell culture sample? How to homogenize the sample prior of heme measurement?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How does this kit work with cell culture sample? How do you homogenize the cells prior of measurement?
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This kit was validated for use with serum, plasma and whole blood. Cell lysates have not been analyzed with this kit and no specific guidance is provided for this type of sample preparation.
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Could I use distilled water instead of ultrapure? Can I use this kit to measure how much plasma free hemoglobin I have in a plasma sample?
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Only ultra pure water should be used because it is filtered through a 0.22 μm filter which removes any bacteria that could interfere with the chemical reaction.
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Does MAK316 work for mouse sera?
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MAK316 is expected to work for all species and should be compatible with mouse sera samples, as well as with other sample types such as blood, serum, plasma, and urine.
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how is this kit used
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The kit is used according to the product protocol. Here is a link to that document:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/386/102/mak316bul.pdf
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お世話になります。臨床研究でヒトの血栓を回収してホルマリン固定しております。遊離ヘムを染めたいと考えておりますが 可能でしょうか？また、検体を送れば染めていただけるのでしょうか？
1 answer-
This product is specifically tested for the quantitative determination of heme in various biological samples. Solid tissues are homogenized and lysed by the heme reagent which contains NaOH and a detergent. This would also apply to the clot sample resulting in the lysis of any intact RBCs. Given the principle of the kit, measuring only the free heme would not be possible. Currently, there is no product available to meet your requirements. Unfortunately, lab services are not offered for this particular application.
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