The calibrator, when diluted for use, has a concentration of 100 mg/dL (100 mg / 100 ml). The kit's Technical Bulletin includes instructions for using the kit with cuvettes as well as with a 96-well plate.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/242/081/mak115bul.pdf
推薦產品
用途
sufficient for 250 colorimetric tests
檢測方法
colorimetric
相關疾病
hematological disorder
儲存溫度
2-8°C
基因資訊
human ... HBA1(3039) , HBA2(3040) , HBB(3043)
mouse ... HBB(15129)
rat ... HBB(24440)
一般說明
特點和優勢
兼容高通量的操作系统。 可在比色皿中使用。
適合性
适于检测血液、血清、血浆和尿液等多种样品中的血红蛋白成分。 本试剂盒可用于研究各类化合物对血红蛋白代谢和表达水平的调控作用。
原則
该分析是根据改进Triton™[NP1]/NaOH方法，血红细胞被转化成比色产物，在400nm测量。该分析在96孔板分析中检测范围为 0.9-200 mg/dL。
法律資訊
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
訊號詞
Warning
危險聲明
危險分類
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1
儲存類別代碼
8B - Non-combustible corrosive hazardous materials
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分析證明 (COA)
Lot/Batch Number
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What is the concentration of the calibrator that comes with the kit? Can this assay be used with cuvettes and if so is there a protocol for reagent volumes and concentrations?
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Is this a hemolysis kit? Certain capmounds are used to detect compatibility with human blood; is this used to detect the amount of free hemoglobin? Can this be used to find whether our compound has cytotoxicity towards RBC?
1 answer-
The assay kit can be used to detect free Hemoglobin. It is not suitable for use in the detection of substances that are cytotoxic to red blood cells.
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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Is there stability information available for the Reagent (MAK115A) and Calibrator (MAK115B) included in the hemoglobin assay kit, especially after they are opened?
1 answer-
The recommended storage for both components is at 2-8 degrees Celsius when not in use. Cycling the components between refrigerated and room temperature may decrease shelf life, but there is no predicted rate of degradation. For prolonged shelf life with frequent use, aliquoting the reagents into smaller tubes for individual use is advised.
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What specific items are included in this kit? Is there a standardized hemoglobin assay?
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There is a hemoglobin calibrator included with the kit, and the concentration of the sample is determined relative to the known concentration of the calibrator.
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