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MAK126

Sigma-Aldrich

胆红素检测试剂盒

sufficient for 180 colorimetric tests

同義詞:

类胆红素检测试剂盒

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84
暫時無法取得訂價和供貨情況

用途

sufficient for 180 colorimetric tests

檢測方法

colorimetric

相關疾病

hematological disorder; gastrointestinal diseases

儲存溫度

2-8°C

相關類別

一般說明

胆红素，也称类胆红素，是由肝脏中血红素分解代谢而形成的降解产物。胆红素在血流中以未结合的不溶形式（间接胆红素）或可溶的葡糖苷酸-结合形式（直接胆红素）循环。结合胆红素从肝脏的胆小管转移到胆囊，在消化过程中被分泌到小肠中。胆红素水平过高可导致黄疸，并表明可能患有肝脏疾病、血液疾病或胆管阻塞。

應用

胆红素检测试剂盒用于检测样品中总胆红素的浓度。[1][2]

特點和優勢

兼容高通量操作系统。经调整可用比色皿进行操作。

適合性

可用于血清和血浆样品中总胆红素和共轭胆红素的测定。

原則

该检测方法基于Jendrassik-Grof法，利用胆红素与重氮化对氨基苯磺酸反应生成可在530 nm处进行检测的比色产物，其与样品中存在的胆红素成比例。该检测试剂盒可检测总胆红素和结合胆红素。

儲存類別代碼

12 - Non Combustible Liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
126CE12A23
126CE12A23
126CE07A16
126CE05A09
126CE05A10

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Zhi-Ming Ding et al.
Frontiers in pharmacology, 9, 410-410 (2018-05-17)
Understanding of the temporal changes of hepatic lesions in the progression and regression of non-alcoholic steatohepatitis (NASH) is vital to elucidation of the pathogenesis of NASH, and critical to the development of a strategy for NASH pharmacotherapy. There are challenges
Kimberly J Jasmer et al.
Pigment cell & melanoma research, 33(6), 850-868 (2020-06-20)
Biosynthesis and degradation of heme, an iron-bound protoporphyrin molecule utilized by a wide variety of metabolic processes, are tightly regulated. Two closely related enzymes, heme oxygenase 1 (HMOX1) and heme oxygenase 2 (HMOX2), degrade free heme to produce carbon monoxide
Bin Dong et al.
Journal of lipid research, 58(2), 350-363 (2016-12-13)
The farnesoid X receptor (FXR) plays critical roles in plasma cholesterol metabolism, in particular HDL-cholesterol (HDL-C) homeostasis. Obeticholic acid (OCA) is a FXR agonist being developed for treating various chronic liver diseases. Previous studies reported inconsistent effects of OCA on
Progression and regression of hepatic lesions in a mouse model of NASH induced by dietary intervention and its implications in pharmacotherapy.
Ding ZM, et al.
Frontiers in Pharmacology, 9, 410-410 (2018)
Regulation of lipid metabolism by obeticholic acid in hyperlipidemic hamsters.
Dong B,et al.
Journal of Lipid Research, 58(2), 350-363 (2017)
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Questions

1–7 of 7 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Can you please confirm me if these assays works with mouse samples?

    1 answer

    1. All three kits have been used with mouse samples.

      Helpful?

  4. What is a typical absorbance range for the 5 mg/dL standard in the MAK126 Bilirubin Assay Kit?

    1 answer

    1. The OD that represents 5mg/dL bilirubin was determined to be 0.52-0.56 during the development of the kit.

      Helpful?

  5. Does this kit work with urine samples?

    1 answer

    1. Kit MAK126 has not been tested on urine samples, so it is not something that can be guaranteed, but it is likely that urine would be okay as long as the concentration falls within the detection range of the kit. Bilirubin does not typically contain urine, so it may not exhibit a response from the kit unless the urine in question comes from a subject with a certain liver condition.

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  6. Can I use the Bilirubin Assay Kit (MK126) to do Bilirubin Assay for mouse?

    1 answer

    1. This Billirubin Assay is not species specific. It has not been tested specifically on mouse serum, however there is no reason to suspect that this kit would not be suitable. Please see the links below to review publications utilizing this product with mouse serum:
      https://doi.org/10.3389/fphar.2018.00410
      https://doi.org/10.1152/ajpheart.00882.2014
      https://doi.org/10.1002/path.5685

      Helpful?

  7. Hola. Deseo medir bilirrubina total en una colección de sueros que se han mantenido congelados a -70ºC varios meses. Necesitaría saber si su kit es recomendable para estas muestras y la cantidad mínima de suero (humano) que se puede usar por reacción. Gcs

    1 answer

    1. Measurement of Total and Direct Bilirubin requires a total of 150 microliters of sample (50 microliters each for Total, Direct, and Sample Blank). Serum (frozen or fresh) is a suitable sample for use with the kit.

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/254/564/mak126bul.pdf

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