This product has been discontinued without a replacement. Technical Service has no data describing its use in testing cell lysates.
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檢測方法
fluorometric
相關疾病
cardiovascular diseases
儲存溫度
−20°C
一般說明
半胱氨酸(CYS)是人体的非必需含硫氨基酸，与胱氨酸有关。 半胱氨酸对于蛋白质合成、解毒和多种代谢功能很重要。 它存在于β-角蛋白中，β-角蛋白是指（趾）甲、皮肤和毛发中的主要蛋白。半胱氨酸对胶原生产，以及皮肤弹性和肌理也很重要。 作为抗氧化剂谷胱甘肽的组分，半胱氨酸在重要生化物质（如辅酶A、肝素和生物素）的代谢中起作用。 半胱氨酸总浓度升高可预测心血管疾病和代谢综合征风险。半胱氨酸缺乏是衰老的后果之一。[1]
特點和優勢
与高通量处理系统兼容。
適合性
适用于检测血清、血浆和其他生物体液中的半胱氨酸。
原則
半胱氨酸检测试剂盒测定各种生物学体液中半胱氨酸的生理浓度。检测原理是还原型半胱氨酸巯基裂解生成荧光产物（激发=365nm/发散=450nm），与样品中的总半胱氨酸含量成正比。该试剂盒具有特异性，其他巯基氨基酸不会造成干扰。该高通量适用性检测试剂盒简单灵敏，可在多种样本中检测到10 µM的半胱氨酸。
儲存類別代碼
10 - Combustible liquids
閃點（°F）
188.6 °F - closed cup
閃點（°C）
87 °C - closed cup
Oxidative stress and ageing: is ageing a cysteine deficiency syndrome?
Droge W.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 360(1464), 2355-2372 (2005)
Kazuki Hayashima et al.
The Journal of biological chemistry, 298(3), 101703-101703 (2022-02-13)
Ferroptosis is an iron-dependent mode of cell death caused by excessive oxidative damage to lipids. Lipid peroxidation is normally suppressed by glutathione peroxidase 4, which requires reduced glutathione. Cystine is a major resource for glutathione synthesis, especially in cancer cells.
Virender Kumar Pal et al.
eLife, 10 (2021-11-19)
A fundamental challenge in human immunodeficiency virus (HIV) eradication is to understand how the virus establishes latency, maintains stable cellular reservoirs, and promotes rebound upon interruption of antiretroviral therapy (ART). Here, we discovered an unexpected role of the ubiquitous gasotransmitter
Xinbo Wang et al.
Cancer research, 81(20), 5217-5229 (2021-08-14)
Ferroptosis is a lipid peroxidation-dependent cell death caused by metabolic dysfunction. Ferroptosis-associated enzymes are promising therapeutic targets for cancer treatment. However, such therapeutic strategies show limited efficacy due to drug resistance and other largely unknown underlying mechanisms. Here we report
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is this product suitable for cell lysate? If possible, i need protocol to prepare sample(cell number, how to lysis etc.)
1 answer-
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Could N-acetyl Cysteine interfere with the MAK255-1KT Cysteine Assay Kit (Fluorometric)? Additionally, would residual H2S also interfere with the assay?
1 answer-
Since the assay principle is based on the cleavage of the thiol group in reduced Cysteine, N-acetyl Cysteine, which contains the thiol group, is likely to be included in the quantification of cysteine present in the biological fluids by the kit. Additionally, the assay should detect Hydrogen sulfide generated by the enzymatic metabolism of Cysteine. Therefore, sulfide salts (such as Sodium sulfide) as well as residual Hydrogen sulfide in the sample would likely be a significant source of interference.
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Are the standards run in kinetic or endpoint mode? How are they plotted?
1 answer-
The standards are run in kinetic mode as well. It's important that the delta T (T2-T1) remains consistent for both the standards and the samples, and in all cases, it should fall within the linear range of the plot. For instance, samples could be read from T=2 to T=4 (assuming linearity) and the standards could be read from T=3 to T=5 (again, assuming linearity), ensuring that the delta T remains the same. Subsequently, the delta RFU is plotted for the standards against concentration, and then the samples can be plotted using their delta RFU to determine the concentration.
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Is the product Cysteine Assay Kit (Fluorometric) MAK255 suitable for use with blood plasma?
1 answer-
The Cysteine Assay Kit (Fluorometric) MAK255 is suitable for use with mammalian plasma samples. Centrifugation of plasma samples is not necessary, as they can be used directly. While the choice of anticoagulant would not make a significant difference, it is generally recommended to avoid EDTA or citrate, which are chelators, and to use heparin instead.
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