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MAK151

Sigma-Aldrich

荧光硫醇测定试剂盒

sufficient for 200 fluorometric tests

同義詞:

Thiol Fluorescent Assay Kit

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About This Item

EC號碼:
分類程式碼代碼:
12161503
NACRES:
NA.84

用途

sufficient for 200 fluorometric tests

檢測方法

fluorometric

相關疾病

endocrinological disorders, diabetes; cancer

儲存溫度

−20°C

一般說明

在游离半胱氨酸、谷胱甘肽(GSH)和蛋白质半胱氨酸残基中发现的硫醇基团可参与许多生物过程。当两个半胱氨酸残基的硫醇基团被氧化时,所产生的二硫键有助于蛋白质的三级或四级结构。

應用

荧光硫醇测定试剂盒已用于测定样品中以硫醇表示的脂质过氧化。

適合性

该试剂盒适用于检测各种不同生物样品中的硫醇,包括血浆、尿液和细胞提取物。该试剂盒未针对测定蛋白质中的硫醇基团进行优化。

原則

荧光硫醇定量试剂盒可提供超灵敏的测定,可用于定量小分子中的硫醇含量。专有的硫醇感受分子在与含硫醇的化合物反应时可产生强荧光加合物(λex = 490/λem = 520 nm)。此外,硫醇加合物的吸收和发射光谱均与pH无关,使得该测定试剂盒非常稳定。该试剂盒可在100 μL测定体积(10 nM).内检测低至1 皮摩尔的半胱氨酸或GSH。可使用96或384多孔板形式方便地进行测定。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


分析證明 (COA)

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Protein sumoylation is one of the most important post-translational modifications regulating many biological processes (Flotho A & Melchior F. 2013. Ann Rev. Biochem. 82:357-85). Our previous studies have shown that sumoylation plays a fundamental role in regulating lens differentiation (Yan

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