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MAK085

Sigma-Aldrich

脂类过氧化作用(MDA)检测试剂盒

sufficient for 100 colorimetric or fluorometric tests

同義詞:

MDA检测, TBARS检测, 硫代巴比妥酸反应底物检测

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84
暫時無法取得訂價和供貨情況

用途

sufficient for 100 colorimetric or fluorometric tests

藥物控制

regulated under CDSA - not available from Sigma-Aldrich Canada

檢測方法

colorimetric
fluorometric

相關疾病

endocrinological disorders, diabetes; neurological disorders; cancer; cardiovascular diseases

儲存溫度

−20°C

一般說明

脂质过氧化是由氧化损伤造成的脂质降解，是氧化应激的重要标志。多不饱和脂质通常易受活性氧的氧化攻击，引起明确的链反应并生成最终产物，如丙二醛(MDA)。脂质过氧化影响许多疾病的病理，包括动脉粥样硬化、糖尿病和阿尔茨海默氏病。

應用

脂质过氧化（MDA）检测试剂盒已用于确定丙二醛（MDA）的水平。[1][2]

適合性

适于测定组织、细胞和血浆等各类样品中的丙二醛(MDA)

原則

本试剂盒通过MDA与硫代巴比妥酸（TBA）反应形成的比色(532 nm)/荧光 (λex = 532/λem = 553 nm) 产物测定脂质过氧化，产物与存在的MDA成比例关系。

相關產品

產品號碼
描述
訂價

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1C

儲存類別代碼

3 - Flammable liquids

閃點（°F）

57.2 °F - closed cup

閃點（°C）

14 °C - closed cup

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
8L03K07390
8A20K07390
8F15K07390
8H25K07390
8C28K07390

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To mitigate cyanobacterial blooms, the naphthoquinone derivative, NQ 2-0, which has selective algicidal activity against cyanobacteria, has been developed. However, due to a lack of information on its algicidal mechanisms, there are significant gaps in our understanding of how this
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Maria I Ayuso et al.
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There is a need to develop additional effective therapies for ischemic stroke. Nitrones, which were first developed as reactive oxygen species (ROS)-trapping compounds, have been proposed as neuroprotective agents for ischemic stroke, a ROS-related disorder. The previous reported ROS-trapping compound
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Xu T, et al.
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Questions

1–7 of 7 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is the MAK085-1KT Lipid Peroxidation (MDA) Assay Kit compatible with tissue samples stored in 4% paraformaldehyde PBS?

    1 answer

    1. The assay has not been tested in PFA-fixed or methanol-fixed samples, and it is not predicted that the kit would work robustly or consistently in these samples.

      Helpful?

  4. Is MAK085 suitable for use on plant samples?

    1 answer

    1. The MAK085 kit has never been tested on plant samples, and thus there is no optimized protocol for plant samples. This kit has only been validated for tissue, cells, serum, or plasma samples. It is possible to adapt the protocol for plant samples and test it, but there is no guarantee that it will work.

      Helpful?

  5. We are planning an MDA assay using mouse tissue. Should I avoid cryopreservation of the samples? How should we store the samples if they cannot be assayed immediately?

    1 answer

    1. Whenever possible, It is recommended to use fresh samples. If sufficient fresh samples are not available, samples may be flash frozen in liquid N2 and stored at -80C. A storage time frame has not been determined. Cryopreservation of samples should be avoided as this may affect the integrity biomolecules and contribute to oxidative stress.

      Helpful?

  6. Can you please send us a protocol how to use MDA Assay kit (MAK085-1KT). We have ordered a kit with lot# 8L03K07390. Thanks

    1 answer

    1. Please see the link below to review the product datasheet which includes the protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/779/633/mak085pis-ms.pdf

      Helpful?

  7. MDA assay kit 구성품 중 TBA(Catalog Number MAK085D)이 4 bottles 있다고 명시되어있는데, 1 bottle 당 몇 g이 들어가있는건가요?

    1 answer

    1. Each bottle contains 250 mg of Thiobarbituric Acid (TBA).

      Helpful?

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