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CAT100

Sigma-Aldrich

过氧化氢酶检测试剂盒

sufficient for ≥100 tests enzymatic, determination of catalase activity in tissues and cells

同義詞:

过氧化氢酶活性检测试剂盒

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84
暫時無法取得訂價和供貨情況

品質等級

200

用途

sufficient for ≥100 tests enzymatic

檢測方法

colorimetric

運輸包裝

wet ice

儲存溫度

2-8°C

基因資訊

human ... CAT(847)

相關類別

一般說明

过氧化氢酶活性检测试剂盒包含研究多种组织和亚细胞组织中过氧化氢酶所需的所有组分。

應用

适用于比色法和紫外分光光度法检测

生化／生理作用

过氧化氢酶是一种普遍存在的抗氧化酶，其功能是催化过氧化氢(H2O2) 的分解，生成水和氧气。过氧化氢在真核细胞内以多种氧化酶和超氧化歧化酶的副产物形式存在。过氧化氢在细胞内的聚集会引发细胞内DNA，蛋白质和脂质的氧化，从而导致基因突变和细胞死亡。过氧化氢酶可去除细胞内的H2O2，保护活细胞免受氧化损伤，过氧化氢酶在氧化胁迫相关疾病中的作用已得到了广泛研究。

特點和優勢

  • 用于检测过氧化氢酶活性 - 可能用于各种不同组织和细胞
  • 简单，优化的实验步骤 - 简单的比色法检测过氧化氢酶体富集和过氧化氢酶活性

適合性

适于研究各种组织和亚细胞器中的过氧化氢酶活性。

原則

该测定方法旨在测定过氧化氢酶作用后残留的过氧化氢底物。首先，过氧化氢在过氧化氢酶作用下转化为水和氧气（过氧化氢酶途径），然后通过叠氮化钠终止这一酶促反应。随后通过比色法，分析反应混合物等分试样中余下的过氧化氢量。10 比色法使用取代苯酚（3,5-二氯-2-羟基苯磺酸），在存在过氧化氢和辣根过氧化物酶（HRP）的条件下，通过氧化作用偶联至4-氨基安替比林中，生成吸收波长520 nm的红色醌亚胺染料（N-（4-安替比林基）-3-氯-5-磺酸酯对苯醌单亚胺）。

單位定義

在pH 7.0，25℃，过氧化氢底物浓度50mM条件下，一单位过氧化氢酶每分钟可以分解 1μmol 过氧化氢生成氧气和水。

準備報告

使用超纯水配置所有溶液。

套裝中的組件也可單獨購買

產品號碼
描述
SDS
  • P6782Peroxidase from horseradish, Type VI-A, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol), 950-2000 units/mg solid (using ABTS) 5 mgSDS
  • 323381Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H2OSDS

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000389380
0000389380
0000198880
0000198880
0000160559

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A J Kowaltowski et al.
FEBS letters, 473(2), 177-182 (2000-05-17)
The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae
M E Tome et al.
Cancer research, 61(6), 2766-2773 (2001-04-06)
Glucocorticoids are used for the treatment of lymphoid neoplasms, taking advantage of the well-known ability of these compounds to cause apoptosis in lymphoid tissues. Previously, we have shown that dexamethasone, a synthetic glucocorticoid, causes a down-regulation of several antioxidant defense
S Tada-Oikawa et al.
FEBS letters, 442(1), 65-69 (1999-01-29)
Pulsed field gel electrophoresis showed that the initiation time of DNA breakage induced by the DNA alkylating agent duocarmycin A, which is not a redox-cycling agent, was almost the same in the human leukemia cell line HL-60 and its H2O2-resistant
Susmita Das Nishu et al.
PloS one, 14(3), e0213370-e0213370 (2019-03-13)
Algicidal bacteria have received broad acceptance as an ecofriendly tool for controlling harmful algal blooms. However, their practical application is still limited to the lab-scale tests due to the complex alga-bacterium interactions in different nutrient statuses. In this study, the
M Zámocký et al.
Progress in biophysics and molecular biology, 72(1), 19-66 (1999-08-14)
This review gives an overview about the structural organisation of different evolutionary lines of all enzymes capable of efficient dismutation of hydrogen peroxide. Major potential applications in biotechnology and clinical medicine justify further investigations. According to structural and functional similarities
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文章

细胞器分离

亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

線粒體壓力和 ROS

細胞氧化壓力是由酵素清除劑和抗氧化調節劑對抗活性氧的損害。

Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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