The M2 Flag antibody is linked to the resin through a covalent linkage to one heavy chain. However, during the conjugation process, some Flag antibodies may also become bound to the resin through noncovalent attachments. To mitigate this, it is recommended to wash the resin using 0.1M Glycine followed by equilibration before use. This washing step ensures that the noncovalently linked antibodies are washed off. The protocol for this product can be found on the product page under more documents, where the Product Information sheet is available.
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推薦產品
共軛
magnetic beads
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
M2, monoclonal
形狀
suspension
儲存期限
2 yr at -20 °C
分析物化學類別
proteins
技術
affinity chromatography: suitable
immunoprecipitation (IP): suitable
珠尺寸
20-75 μm
基質
superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.
同型
IgG1
容量
≥0.6 mg/mL binding capacity
運輸包裝
wet ice
儲存溫度
−20°C
尋找類似的產品？ 前往 產品比較指南
一般說明
抗-FLAG M2磁珠是可与抗-FLAG M2（小鼠单克隆）抗体结合的4％琼脂糖微珠。 该M2抗体可识别FLAG序列的N-末端、Met N-末端和C-末端。 这可实现对含有FLAG肽序列的融合蛋白的检测和捕获。
應用
特點和優勢
该磁性属性可实现：
- 极为快速的分离
- 显著被加速的操作，例如重复的洗涤
-以板式进行多个样本的处理
该可导致：
- 更为快速的实验
- 更好的可重复性
- 目标蛋白更为准确的定量
- 极为快速的分离
- 显著被加速的操作，例如重复的洗涤
-以板式进行多个样本的处理
该可导致：
- 更为快速的实验
- 更好的可重复性
- 目标蛋白更为准确的定量
外觀
以含10mM磷酸钠、150mM 氯化钠（pH 7.4）、 0.02%（w/v）叠氮化钠（PBA/A）的50%甘油的50%悬浮液供应
其他說明
法律資訊
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
FLAG™亲和凝胶、FLAG™ 标签、3x FLAG™标签、DYKDDDDK标签
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相關產品
產品號碼
描述
訂價
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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文章
The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.
FLAG®表达系统是一种表达、纯化和检测重组融合蛋白的成熟方法。作为FLAG®的可靠供应商，Sigma®现提供FLAG标签抗体标记的免疫磁珠，用于免疫沉淀、蛋白纯化和蛋白互作研究。ANTI-FLAG® M2磁珠由ANTI-FLAG® M2鼠单克隆抗体与超顺磁铁包覆的4%琼脂糖微珠偶联而成，平均粒径50 µM。M2抗体可结合哺乳动物、细菌和植物提取物融合蛋白N端、Met-N端或C端的FLAG®肽。
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
使用抗 FLAG® M2 磁珠小規模純化 FLAG® 標籤蛋白的洗脫技術比較。
相關內容
蛋白質純化技術、試劑，以及使用離子交換法、尺寸排阻法和蛋白質親和層析法等方法純化重組蛋白質的方案。
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
適用於各種表達系統的蛋白質表達技術，支援研究、治療和疫苗生產。
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
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Bonjour Je souhaiterais que vous me confirmiez que les billes Billes magnétiques Anti-FLAG® M2 sont bien directement couplées à l'Anticorps anti flag ? Pouvez vous également me fournir un protocole d'immunocapture utilisant ces billes s'il vous plait ?
1 answer-
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Which antibody is used to bind to the beads in Anti-FLAG® M2 Magnetic Beads(M8823) and EZview™ Red ANTI-FLAG® M2 Affinity Gel(F2426)? I have ordered the antibody(F1804 Monoclonal ANTI-FLAG® M2 antibody produced in mouse). Is it this one?
1 answer-
The antibody used for these items is proprietary information. ANTI-FLAG M2 Magnetic Beads consist of a mouse-derived, ANTI-FLAG M2 monoclonal antibody attached to superparamagnetic iron-impregnated 4% agarose beads. EZview Red ANTI-FLAG M2 Affinity Gel contains ANTI-FLAG M2 monoclonal antibody covalently attached to cross-linked 4% agarose beads. The ANTI-FLAG M2 antibody recognizes the FLAG octapeptide sequence (N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C) at the N-terminus, Met-N-terminus, or C-terminus locations of a fusion protein.
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Hello, I want to ask how many Flag-antibody on those beads?
1 answer-
The specific number of antibodies per bead is not determined. The minimum amount of antibody in this product is 2.5 mg/mL of suspension. A range of 1.7 - 2.1 mL of suspension will yield 1 mL of packed resin. Both of these values are lot specific and reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/m8823#product-documentationHelpful?
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What is the maximum number of times that the ANTI-FLAG® M2 Magnetic Beads (M8823) can be reused?
What is the maximum number of times that the ANTI-FLAG® M2 Magnetic Beads (M8823) can be reused?
1 answer-
The maximum number of times that the ANTI-FLAG® M2 Magnetic Beads (M8823) can be reused varies depending on the specific sample applied. It can range from one to twenty uses. Factors such as the cleanliness of the sample and the presence of substances that may clog the resin or cause nonspecific binding can affect the number of reuses. In typical scenarios, the beads are commonly reused for 3-5 times. It is essential to refer to the technical bulletin for specific situations where the beads cannot be reused, such as when using reagents containing SDS: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/075/m8823bul-ms.pdf
Helpful?
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Are the M2 antibodies on the Anti-FLAG® M2 Magnetic Beads covalently bound?
1 answer-
The M2 Flag antibody is linked to the resin through a covalent linkage to one heavy chain. However, during the conjugation process, some Flag antibodies may also become bound to the resin through noncovalent attachments. To mitigate this, it is recommended to wash the resin using 0.1M Glycine followed by equilibration before using it. This washing step ensures that the noncovalently linked antibodies are washed off.
Helpful?
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Is there a protocol available for using the Anti-Flag M2 Magnetic Beads in a ChIP-Seq assay? Additionally, if the lysis buffer contains SDS and sodium deoxycholate at a concentration of 0.1%, will the presence of both denature or interfere with binding in the research process?
1 answer-
The use of Anti-Flag M2 Magnetic Beads in a ChIP-Seq assay has not been validated. However, according to a citation provided in Stem Cells (PMID: 31348575), it suggests that these beads are compatible for such applications. Please note that M8823 is not recommended for use with Sodium Dodecyl Sulfate (SDS) or deoxycholate, as these components can denature the immobilized antibody and disrupt its binding to FLAG fusion proteins.
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Does M8823 have the capability to detect internal FLAG tags in addition to N and C terminal sequences?
1 answer-
This paper (Zordan RE, Beliveau BJ, Trow JA, Craig NL, Cormack BP. Avoiding the ends: internal epitope tagging of proteins using transposon Tn7. Genetics. 2015 May;200(1):47-58. doi: 10.1534/genetics.114.169482. Epub 2015 Mar 5. PMID: 25745023; PMCID: PMC4423380) indicates that internal FLAG epiptopes were inserted into ORFs in yeast and detected by our F1804 antibody via Western blot. The biggest issue to overcome is the availability of the epitope for the antibody. If it is buried where it is not accessible in its native form, may not be possible. Denaturing the protein at least in this situation shows that the antibody can detect the protein.
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Is Product No. CL4B200, Sepharose® CL-4B a suitable alternative for a negative bead control if magnetic beads without FLAG are not available? Are there any other recommendations for this purpose?
1 answer-
For a negative control bead, any underivatized bead can essentially be used. While the Anti-FLAG® M2 Magnetic Beads (M8823) will work as a negative bead, it may not be suitable for magnetic applications. If a magnetic format is required, it is recommended to use polystyrene-based magnetized beads such as Product No. 49664, Micro particles based on polystyrene, magnetic. Alternatively, another option is to create a negative control using a non-FLAG tagged protein. This control can demonstrate the specificity of M8823 by showing its significant detection of FLAG-tagged proteins.
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Are there any problems with incubation at 40°C for this product?
1 answer-
The information you are requesting can be supplied by our Technical Service team who can assist you further. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
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Dear all, I have a question regarding one of you product: Anti-FLAG® M2 Magnetic Beads, Cat. No: M8823. Is 0.1% SDS compatible with product binding FLAG peptide?
1 answer-
This product is not recommended for use with Sodium Dodecyl sulfate nor deoxycholate as they are known to denature the immobilized antibody and interfere with antibody binding to FLAG fusion protein.
Regarding reagent compatibility and protocol information, please see page 5 of the product technical bulletin at the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/075/m8823bul-ms.pdfHelpful?
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