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F4799

Sigma-Aldrich

3xFLAG™ Peptide

≥90% (HPLC/MS), lyophilized powder

同義詞:

Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk肽, dykddddk肽

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About This Item

經驗公式（希爾表示法）:
C120H169N31O49S
分子量：:
2861.87
MDL號碼:
MFCD01863911
分類程式碼代碼:
12352202
NACRES:
NA.32

product name

3X FLAG® 肽, lyophilized powder

化驗

≥90% (HPLC/MS)

品質等級

200

形狀

lyophilized powder

運輸包裝

wet ice

儲存溫度

2-8°C

相關類別

一般說明

3X FLAG肽是一种具有23个氨基酸残基的合成肽。在该肽中Asp-Tyr-Lys-Xaa-Xaa-Asp基序重复了三次。在C-末端的八个氨基酸组成了经典的FLAG序列(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)。
Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gel.

應用

用于从在溶液中的或结合至抗-FLAG® M2琼脂糖亲和凝胶（A2220）上琼脂糖上的抗-FLAG ®M2单克隆抗体（F1804）进行3X FLAG®融合蛋白的竞争性洗脱。通过亲和色谱法可实现这一目标。用于将3X FLAG融合蛋白从抗-FLAG® M2亲和凝胶上温和的洗脱。1X FLAG肽（F3290）将不会把3X FLAG融合蛋白从抗-FLAG®亲和凝胶上洗脱下来。


请访问我们的FLAG® 应用 门户网站，了解更多产品详情。

準備報告

如需制备储备溶液，可溶解于TBS（含有150 mM NaCl的50mM Tris-HCl，pH7.4）至5 mg/mL的浓度。

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

產品號碼
描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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存取文件庫

Andrew T Schiffmacher et al.
The Journal of cell biology, 215(5), 735-747 (2016-11-20)
During epithelial-to-mesenchymal transitions (EMTs), cells disassemble cadherin-based junctions to segregate from the epithelia. Chick premigratory cranial neural crest cells reduce Cadherin-6B (Cad6B) levels through several mechanisms, including proteolysis, to permit their EMT and migration. Serial processing of Cad6B by a
Paul L Colbert et al.
Oncotarget, 6(2), 953-968 (2014-12-02)
Microtubules (MTs) are components of the cytoskeleton made up of polymerized alpha and beta tubulin dimers. MT structure and function must be maintained throughout the cell cycle to ensure proper execution of mitosis and cellular homeostasis. The protein tyrosine phosphatase
Yannick Doyon et al.
Molecular and cellular biology, 24(5), 1884-1896 (2004-02-18)
The NuA4 histone acetyltransferase (HAT) multisubunit complex is responsible for acetylation of histone H4 and H2A N-terminal tails in yeast. Its catalytic component, Esa1, is essential for cell cycle progression, gene-specific regulation and has been implicated in DNA repair. Almost
Min Hwa Shin et al.
PLoS genetics, 12(2), e1005884-e1005884 (2016-03-02)
The inactivation of p53 creates a major challenge for inducing apoptosis in cancer cells. An attractive strategy is to identify and subsequently target the survival signals in p53 defective cancer cells. Here we uncover a RUNX2-mediated survival signal in p53
Lingyan Wang et al.
Nature communications, 8, 13876-13876 (2017-02-09)
Cellular protein interaction networks are integral to host defence and immune signalling pathways, which are often hijacked by viruses via protein interactions. However, the comparative virus-host protein interaction networks and how these networks control host immunity and viral infection remain
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文章

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

抗FLAG® M2 磁珠

使用抗 FLAG® M2 磁珠小規模純化 FLAG® 標籤蛋白的洗脫技術比較。

條款

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

使用單株抗體親和膠對 FLAG 融合蛋白進行免疫沉淀

使用 M2 單株抗體 4% 琼脂糖親和膠對 FLAG 融合蛋白進行免疫沉淀 (IP) 的規程

相關內容

蛋白質純化

蛋白質純化技術、試劑，以及使用離子交換法、尺寸排阻法和蛋白質親和層析法等方法純化重組蛋白質的方案。

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

蛋白質表達

適用於各種表達系統的蛋白質表達技術，支援研究、治療和疫苗生產。

Protein Expression

Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

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