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Merck
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A9594

Sigma-Aldrich

单克隆抗-FLAG® M2-Cy3™ 小鼠抗

clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

同義詞:

单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

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About This Item

分類程式碼代碼:
41106514
NACRES:
NA.32

生物源

mouse

共軛

CY3 conjugate

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

M2, monoclonal

形狀

buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

物種活性

all

濃度

~1 mg/mL

技術

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

同型

IgG1

免疫原序列

DYKDDDDK

運輸包裝

dry ice

儲存溫度

−20°C

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相關類別

一般說明

单克隆抗标记 M2-Cy3（小鼠IgG）抗体与花菁染料 Cy3 共价偶联。抗体缀合物结合FLAG融合蛋白，并且将在FLAG融合蛋白的N末端、Met-N末端或C末端以识别FLAG序列。

應用

实现简单的一步式免疫细胞化学检测。 特别适用于小鼠宿主表达FLAG融合蛋白的检测，这种检测使用抗小鼠二抗可能会发生交叉反应。

请访问我们的FLAG®应用页面，了解更多产品详情。
此抗体已成功使用的应用，以及相关的同行评审论文如下。
蛋白免疫分析（Western Blotting）（1篇文章）

外觀

溶于磷酸盐缓冲盐水，含有 1%BSA 和防腐剂

準備報告

用 Tris 缓冲盐水 (TBS) 稀释抗体：0.05 MTris，pH 7.4，含 0.15 M NaCl。

其他說明

免疫细胞化学的建议浓度为1-10 mg / ml。

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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