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F2426

Millipore

EZview™ Red 抗-FLAG® M2 亲和凝胶

clone M2

同義詞:

单克隆抗-FLAG® M2 小鼠抗, 抗 ddddk, 抗 dykddddk

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.32

無性繁殖

M2, monoclonal

品質等級

200

分析物化學類別

proteins

技術

affinity chromatography: suitable
immunoprecipitation (IP): suitable

基質

4% agarose bead; 45-165μm bead size

同型

IgG1

容量

≥0.6 mg/mL, gel binding capacity

運輸包裝

wet ice

儲存溫度

−20°C

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相關類別

一般說明

EZview Red 抗-FLAG M2 亲和凝胶是含有抗FLAG M2抗体的树脂，共价交联在4%的琼脂糖珠上。该亲和凝胶用于将标记融合蛋白与样品结合，如细胞裂解物和组织，用于纯化标记蛋白，用于制备免疫沉淀分析。红色染料增强了可视性，以获得更有效的结果。琼脂糖珠结合于N-端、MET-N端和C-端FLAG融合蛋白、3xFLAG标记融合蛋白。

特異性

适用于纯化N-端、MET-N端和C-端FLAG融合蛋白、3xFLAG标记融合蛋白。

應用

FLAG-和3xFLAG-标签融合蛋白的免疫沉淀（IP）。

洗脱-FLAG 多肽，甘氨酸，pH 3.5，3x FLAG 多肽

在我们的 FLAG® 应用门户了解更多产品详情。

外觀

1:1 (v/v) 悬浮于含50%的甘油和15 ppm Kathon的PBS中

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

FLAG® 亲和凝胶，FLAG®标签，3xFLAG®标签，DYKDDDDK标签

相關產品

產品號碼
描述
訂價

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000432486
0000432486
0000269913
0000272448
0000278660

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存取文件庫

Kara R Jones et al.
Molecular cancer therapeutics, 4(10), 1541-1547 (2005-10-18)
Incomplete DNA repair or misrepair can contribute to the cytotoxicity of DNA double-strand breaks. Consequently, interference with double-strand break repair, by pharmacologic or genetic means, is likely to sensitize tumor cells to ionizing radiation. The current studies were designed to
Matthew S Walters et al.
Journal of virology, 84(13), 6861-6865 (2010-04-16)
Varicella zoster virus encodes an immediate-early (IE) protein termed ORF61p that is orthologous to the herpes simplex virus IE protein ICP0. Although these proteins share several functional properties, ORF61p does not fully substitute for ICP0. The greatest region of similarity
Jiahai Zhou et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(39), 14343-14348 (2006-09-16)
The unfolded protein response (UPR) is an evolutionarily conserved mechanism by which all eukaryotic cells adapt to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). Inositol-requiring kinase 1 (IRE1) and PKR-related ER kinase (PERK) are two type I
W Du et al.
Cell death and differentiation, 16(11), 1493-1504 (2009-07-11)
The tumor suppressor p53 induces potent anti-proliferative responses in stressed cells; in unstressed cells this ability of p53 is restrained by Hdm2. Expression of Hdm2 is also induced by p53, thereby establishing feedback inhibition. Regulation of the p53-Hdm2 interaction and
Ting-Ting Qu et al.
BMC cancer, 18(1), 27-27 (2018-01-06)
Lin28B and its paralog Lin28A are small RNA binding proteins that have similar inhibitory effects, although they target separate steps in the maturation of let-7 miRNAs in mammalian cells. Because Lin28B participates in the promotion and development of tumors mostly
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條款

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

使用單株抗體親和膠對 FLAG 融合蛋白進行免疫沉淀

使用 M2 單株抗體 4% 琼脂糖親和膠對 FLAG 融合蛋白進行免疫沉淀 (IP) 的規程

相關內容

蛋白質純化

蛋白質純化技術、試劑，以及使用離子交換法、尺寸排阻法和蛋白質親和層析法等方法純化重組蛋白質的方案。

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

蛋白質表達

適用於各種表達系統的蛋白質表達技術，支援研究、治療和疫苗生產。

Protein Expression

Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

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