The binding capacity of the resin must be ? 0.6 mg/mL to meet specifications. This capacity will vary from lot to lot.
推薦產品
無性繁殖
M2, monoclonal
品質等級
分析物化學類別
proteins
技術
affinity chromatography: suitable
immunoprecipitation (IP): suitable
基質
4% agarose bead; 45-165μm bead size
同型
IgG1
容量
≥0.6 mg/mL, gel binding capacity
運輸包裝
wet ice
儲存溫度
−20°C
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一般說明
EZview Red 抗-FLAG M2 亲和凝胶是含有抗FLAG M2抗体的树脂，共价交联在4%的琼脂糖珠上。该亲和凝胶用于将标记融合蛋白与样品结合，如细胞裂解物和组织，用于纯化标记蛋白，用于制备免疫沉淀分析。红色染料增强了可视性，以获得更有效的结果。琼脂糖珠结合于N-端、MET-N端和C-端FLAG融合蛋白、3xFLAG标记融合蛋白。
特異性
适用于纯化N-端、MET-N端和C-端FLAG融合蛋白、3xFLAG标记融合蛋白。
應用
外觀
1:1 (v/v) 悬浮于含50%的甘油和15 ppm Kathon的PBS中
法律資訊
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
EZview is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
FLAG™ 亲和凝胶，FLAG™标签，3xFLAG™标签，DYKDDDDK标签
相關產品
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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條款
Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels
使用 M2 單株抗體 4% 琼脂糖親和膠對 FLAG 融合蛋白進行免疫沉淀 (IP) 的規程
相關內容
蛋白質純化技術、試劑，以及使用離子交換法、尺寸排阻法和蛋白質親和層析法等方法純化重組蛋白質的方案。
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
適用於各種表達系統的蛋白質表達技術，支援研究、治療和疫苗生產。
Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.
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What is the binding capacity of the Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, resin?
1 answer-
Helpful?
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When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, should I use a 3X FLAG peptide or a 1X FLAG peptide to elute my protein?
1 answer-
If you have a 3X FLAG-tagged protein, then you will need to use the 3X FLAG peptide. If you have a 1X FLAG-tagged protein, you can use the 1X FLAG peptide or the 3X FLAG peptide. We have not noticed a significant difference in elution efficiency by using a 3X FLAG peptide on a 1X FLAG-tagged protein.
Helpful?
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When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, I see bands at 20-25 kDa and 50-60 kDa appearing in my Westerns that are not my FLAG-tagged protein. How can I prevent this?
1 answer-
As a result of the conjugation, there may be some M2 antibody that is not conjugated to the resin, but is associated with the resin and may appear in acid elutions as heavy and light chain when using the anti-mouse IgG conjugated secondary antibody. We recommend a acid wash (0.1 M glycine-HCL pH 3.5) and neutralization of the resin (do not allow the acid wash to sit on the resin longer than 20 minutes) prior to applying the lysate. Another way to avoid this is to use a directly conjugated FLAG antibody for detection such as product A8592 ant-FLAG M2 HRP, or the rabbit anti-FLAG polyclonal antibody, F7425.
Helpful?
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When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, I have a lot of non-specific proteins that are eluting with my FLAG-tagged protein. How can I get rid of these?
1 answer-
One way to remove non-specific proteins is to pre-bind the protein lysate with unconjugated resin. We recommend product 4B200 for this purpose. Other methods would be to increase the stringency of the washes by increasing salt concentration (the resin can tolerate up to 1M NaCl) or including detergents that are compatible with the resin.
Helpful?
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When using Product F2426, EZview™ Red ANTI-FLAG® M2 Affinity Gel clone M2, how can I elute my protein?
1 answer-
Elution with the peptide is the most gentle method. Acid elution (0.1 M glycine-HCL pH 3.5) is a more stringent method of elution, and should be evaluated for its effect on your protein if it is to be used in downstream applications. Boiling the resin in sample buffer is the most denaturing condition. If this condition is used, the resin cannot be re-used, due to the presence of SDS and/or reducing agents.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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