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一般說明
莫洛尼鼠白血病病毒(M-MLV)逆转录酶从质粒表达M-MLV部分pol基因的大肠杆菌中分离获得。MoMLVRT由671个氨基酸组成。它是一种DNA聚合酶，可通过单链RNA、DNA或RNA-DNA杂合体（使用引物）合成互补的DNA链。
應用
M-MLV逆转录酶已用于：
- 制备cDNA文库或合成第一条cDNA链
- 2步法RT-PCR检测
- 实时定量聚合酶链反应(RT-qPCR)
- 逆转录
特點和優勢
- 热稳定性逆转录酶在37℃时活性佳。
- 可用于制备长达7 kb的第一链cDNA。
包裝
本品为含DTT 的10× M-MLV逆转录酶缓冲液。
單位定義
一单位本品可在10分钟内将1 nmol TTP整合进酸性可沉淀物质中。反应在37℃下进行，以poly(A)：oligo dT为模板：引物。
準備報告
该酶从可在质粒上表达M-MLV pol基因的Escherichia coli 中纯化所得。
法律資訊
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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文章
Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
基因表達分析的挑戰包括 mRNA 的穩定性、時間性轉錄模式以及 mRNA 與蛋白質的相關性，這些都會影響準確性。
相關內容
RT-qPCR 可檢測特定目標，應用於基因表達和病原體檢測。
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
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