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M8537

Sigma-Aldrich

MCDB 131培养基

With ʟ-glutamine, without sodium bicarbonate, powder, suitable for cell culture

同義詞:

131 Medium, Medium 131, Molecular, Cellular, and Development Biology 131 medium

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

product name

MCDB 131培养基, With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

形狀

powder

濃度

11.6 g/L

技術

cell culture | mammalian: suitable

成分

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

運輸包裝

ambient

儲存溫度

2-8°C

一般說明

MCDB-131培养基提供了一个清晰和平衡的营养环境,可选择性地促进特定细胞类型的生长。MCDB-131在补充0.7% FBSP、10 ng/ml表皮生长因子、1 μg/ml氢化可的松后,可支持人微血管内皮细胞(包括人脐静脉内皮细胞)的克隆性生长。MCDB培养基用于特定类型细胞的低蛋白或无血清生长,使用的是激素、生长因子、微量元素或低水平透析胎牛血清蛋白(FBSP)。 

應用

MCDB-131培养基已用于:

  • 作为牛视网膜内皮细胞基础培养基的组分
  • 培养牛肺微血管内皮细胞
  • 分离猪颅内内皮细胞和猪主动脉内皮细胞

數量

按照每升培养基11.6克粉末配制。

重構

补充1.18 g/L碳酸氢钠。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Endocardial endothelial cells (EECs), when compared with endothelial cells of arteries and veins, possess higher resistance to apoptosis-inducing anticancer agents. The mechanism of this resistance property is unknown. We have investigated the molecular mechanism, which contributes to increased cell survival

條款

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

粉狀介質和鹽混合物具有極強的吸濕性,因此應防止大氣中的濕氣進入。

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