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M5650

Sigma-Aldrich

Eagle最低必需培养基

With non-essential amino acids, Earle′s salts and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

同義詞:

MEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

product name

Eagle最低必需培养基, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

Eagle最低必需培养基(MEM)是Harry Eagle开发的人工合成细胞培养基。培养基含有较高浓度的氨基酸,因此比较接近哺乳动物培养细胞的蛋白质成分。在含有Hanks′或Earle′s盐的配方中选择补充非必需氨基酸,可扩大该培养基的用途。通过选择性去除钙成分后,该配方可进一步改良为适合悬浮细胞培养。MEM可用于各种细胞的单层培养。

應用

Eagle最低必需培养基已用于培养:
  • HeLa细胞
  • Madin-Darby犬肾细胞 (MDCK)
  • 软骨肉瘤细胞系

此培养基在血清减量条件下适合贴壁细胞生长,也可作为无血清培养基开发的基础配方。

重構

添加 0.292g/L L-谷氨酰胺。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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We present a strategy for increasing the anatomical realism of organoids by applying asymmetric cues to mimic spatial information that is present in natural embryonic development, and demonstrate it using mouse kidney organoids. Existing methods for making kidney organoids in
Junjie Jing et al.
International journal of clinical and experimental medicine, 8(8), 13127-13135 (2015-11-10)
Tectonic family member 1 (TCTN1) encodes a member of the tectonic family which are evolutionarily conserved secreted and transmembrane proteins, involving in a diverse variety of developmental processes. It has been demonstrated that tectonics expressed in regions that participate in
Bryan Tay et al.
PloS one, 14(3), e0213751-e0213751 (2019-03-12)
Voltage-gated sodium channels (NaVs) are key therapeutic targets for pain, epilepsy and cardiac arrhythmias. Here we describe the development of a no-wash fluorescent sodium influx assay suitable for high-throughput screening and characterization of novel drug leads. Addition of red-violet food
Emanuela Profilo et al.
Biochimica et biophysica acta, 1863(6), 1422-1435 (2017-02-27)
The mitochondrial aspartate-glutamate carrier isoform 1 (AGC1) catalyzes a Ca

文章

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

大量 MEM 配方可供選擇。 由 Harry Eagle 開發的 Minimum Essential Medium (MEM) 是所有合成細胞培養基中使用最廣泛的一種。

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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