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M7145

Sigma-Aldrich

MEM 非必需氨基酸溶液 (100×)

Without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

同義詞:

MEM NEAA

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About This Item

分類程式碼代碼:
12352207
eCl@ss:
32160406
NACRES:
NA.75
暫時無法取得訂價和供貨情況

產品名稱

MEM 非必需氨基酸溶液 (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture

品質等級

200

無菌

sterile-filtered

產品線

BioReagent

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

儲存溫度

2-8°C

相關類別

一般說明

Eagle′s基本必需培养基(MEM)是一种合成培养基，由平衡盐溶液、氨基酸和维生素组成。这些组份为培养细胞生长的必需组份。MEM可添加非必需氨基酸，而制备MEM非必需氨基酸溶液。非必需氨基酸包括L-丙氨酸、L-天冬酰胺、L-天冬氨酸、L-甘氨酸、L-丝氨酸、L-脯氨酸和L-谷氨酸。[1]

應用

向培养基中添加氨基酸补充剂不仅可以刺激细胞生长还可以延长培养细胞的活力。MEM非必需氨基酸溶液的补充物，可增加营养成分并减少 体外细胞的生物合成负担。
MEM非必需氨基酸溶液已可用于维持哺乳动物细胞的Dulbecco′s基本必需培养基。[2]它已被添加到卵母细胞成熟培养基中。[3][4]

生化／生理作用

杂交瘤培养研究表明，Eagle8242;s基本培养基(MEM)添加非必需氨基酸可提高杂交瘤细胞在死亡阶段的膜稳定性。这也会导致死亡阶段抗体分泌增加，乳酸浓度下降。[5]

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

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儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
RNBM1904
RNBM0563
RNBM0097
RNBL7298
RNBL4185

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存取文件庫

Ayako Suzuki et al.
Genome biology, 16, 66-66 (2015-04-19)
To understand the heterogeneous behaviors of individual cancer cells, it is essential to investigate gene expression levels as well as their divergence between different individual cells. Recent advances in next-generation sequencing-related technologies have enabled us to conduct a single-cell RNA-Seq
Excessive concentration of glucose during in vitro maturation impairs the developmental competence of bovine oocytes after in vitro fertilization: relevance to intracellular reactive oxygen species and glutathione contents.
Hashimoto S et al.
Molecular Reproduction and Development, 56, 520-520 (2000)
Cell Culture
Jakoby WB
Methods in Enzymology, 58 (1979)
Influence of non-essential amino acids on apoptotic and necrotic death of mouse hybridoma cells in batch cultures
Fassnacht D
Biotechnology Letters, 19(1), 35-38 (1997)
Lei Xi Chen et al.
The Laryngoscope, 129(9), 2139-2146 (2019-03-19)
Surgical adhesives are increasingly used for vocal fold microsurgery to assist wound closure and reduce the risks of scar formation. Currently used vocal fold adhesives such as fibrin glue, however, have thus far not been found to promote wound closure
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文章

Amino Acid Codon Wheel

Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily.

氨基酸編碼輪

Amino Acid Codon Wheel 用於快速 RNA 翻譯。從您的 RNA 序列快速輕鬆地找出哪個氨基酸被翻譯出來。

條款

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

相關內容

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

Questions

1–4 of 4 Questions  

  1. 안녕하세요. Lot. RNBL4088 제품 유통기한 알 수 있을까요?

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one-year warranty from the date of shipment.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  2. What is the concentration of Product M7145, MEM Non-essential Amino Acids, 100X solution?

    1 answer

    1. M7145 is a 100X solution of amino acids (Ala, Asn, Asp, Glu, Gly, Pro and Ser). The concentration of each of the seven amino acid at 100X is 10 mM; therefore at 1X, the concentration of each of them is 0.1 mM.

      Please see the component chart for more information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/334/224/m7145for.pdf

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Can I store Product M7145, MEM Non-essential Amino Acids, 100X solution at -20°C?

    1 answer

    1. Product M7145 is a 100X solution of non-essential amino acids that should be stored at 2-8 °C for maximum stability. If the product is properly stored and handled, we guarantee the product for one year after it is received. We have not tested the stability of the product at -20°C for long term storage, so we do not recommend aliquoting and freezing the product. It is likely that storage at -20°C will cause precipitation of some of the amino acids upon thawing. If precipitation occurs, the product should not be used.

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