LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
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等級
Molecular Biology
for molecular biology
無菌
non-sterile
形狀
tablet
技術
microbiological culture: suitable
應用
food and beverages
microbiology
儲存溫度
room temp
適合性
nonselective for Escherichia coli
nonselective for coliforms
一般說明
Lennox LB 是一种高度对照的微生物生长培养基，用于培养 大肠杆菌 。这种营养丰富的微生物肉汤含有多肽、氨基酸、水溶性维生素和碳水化合物，呈低盐配方。添加琼脂为微生物生长提供固体培养基。
應用
适用于非选择性培养大肠杆菌菌株，用于克隆、DNA 质粒生产和重组蛋白的生产。也适用于添加适当抗生素时的选择性培养，包括需要低盐条件的抗生素，如 Zeocin ®。
特點和優勢
含琼脂的 Lennox LB 片剂提供：
- 一个预算较低的替代预制板
- 方便小包装，无需称重
- 快速制备 – 无需搅拌
成分
13.72 g/L 琼脂
9.14 g/L 胰蛋白胨
4.57 g/L 酵母提取物
4.57 g/L 氯化钠
1.6 g/L 惰性结合剂
9.14 g/L 胰蛋白胨
4.57 g/L 酵母提取物
4.57 g/L 氯化钠
1.6 g/L 惰性结合剂
重構
将每片 1.68 g 片剂加至 48.3 mL 水中（无需搅拌）。121°C 高压灭菌 20 分钟。在加入抗生素（如需要）之前略微冷却。倒入皮氏培养皿中，待其凝固。
法律資訊
Zeocin is a registered trademark of Cayla Sarl
相關產品
產品號碼
描述
訂價
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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分析證明 (COA)
Lot/Batch Number
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General protocols for growth of competent cells in microbial medium.
培養大腸桿菌的規模從小培養到發酵。包括啟動培養、懸浮細胞、單菌培養和噬菌體 M13 複製的程序。
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What is the difference among the many LB - Lennox products?
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Will adding magnesium to the culture medium increase cell density?
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In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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Which bacterial culture medium is the best choice for my application?
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Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What are the differences among the Luria, Lennox and Miller LB formulations?
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LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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