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S1797

Sigma-Aldrich

SOC培养基

For use in transformation

同義詞:

Super Optimal broth

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100 ML

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About This Item

分類程式碼代碼:
41106200
NACRES:
NA.85

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履行和交付延遲

等級

for molecular biology

品質等級

500

無菌

0.2 μm filtered

形狀

liquid

成份

Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

技術

microbiological culture: suitable

應用

agriculture

儲存溫度

2-8°C

適合性

nonselective for Escherichia coli
nonselective for coliforms

相關類別

一般說明

含有分解代谢抑制物的培养基（SOC）是一种富含葡萄糖的微生物生长培养基，主要用于大肠杆菌感受态细胞转化后的复苏。 在这种营养丰富的微生物培养基中培养大肠杆菌细胞，可以抑制克隆载体上携带的lac启动子的意外激活，从而提高含有毒蛋白基因的重组质粒的转化效率。[1]SOC培养基采用低盐配方，含有多肽、氨基酸、水溶性维生素和葡萄糖。

應用

SOC培养基可用于大肠杆菌DH5α 感受态细胞的转化。 [2] 它还被用于培养克隆后的白色菌落，以鉴定感兴趣的重组蛋白。 [3]

特點和優勢

SOC液体提供：
  • 经过滤灭菌的即用型培养基
  • 便利的包装规格
  • 标准配方

相關產品

產品號碼
描述
訂價

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
SLCN8706
SLCL9800
SLCN2539
SLCF7416
SLCG7766

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存取文件庫

Rebekah M Martin et al.
mSystems, 3(3) (2018-07-03)
Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
Daniel C Volke et al.
Bio-protocol, 11(4), e3917-e3917 (2021-03-19)
Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted
Detection of Cryptosporidium parvum Oocysts on Fresh Produce Using DNA Aptamers
Iqbal A
PLoS ONE (2015)
S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
Qiao-Yang Sun et al.
Analytical biochemistry, 394(1), 144-146 (2009-07-23)
In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which is a glucose-containing rich medium, significantly improved the transformation efficiency of a recombinant plasmid containing a toxic plant subtilase SaSBT1 cDNA.
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文章

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

細菌轉換協議

合格細胞的生長及其轉化（DNA 的吸收）的一般規程。

條款

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

有效細胞協議

關於合格細胞的技術文章。轉化是某些細菌從環境中吸收外來遺傳物質（裸 DNA）的過程。

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

微生物生長協議

培養大腸桿菌的規模從小培養到發酵。包括啟動培養、懸浮細胞、單菌培養和噬菌體 M13 複製的程序。

Questions

1–4 of 4 Questions  

  1. That is the shelf life of SOC medium if kept refrigerated at 4C?

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 2 years after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

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  2. Why is SOC Medium (S1797) used to recover bacterial cells before transformation?

    1 answer

    1. SOC is a rich bacterial broth that also contains glucose, which aids in the recovery process and prepares the cells for efficient transformation.  The use of other media, such as LB - Lennox may also be acceptable, but the recovery and transformation may not be as efficient.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Which bacterial culture medium is the best choice for my application?

    1 answer

    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

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