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一般說明
角蛋白酶是一种非特异性丝氨酸蛋白酶，能切割非末端肽键。这种酶在 37°-70°C 之间有活性。通常在 70°C 时活性最高。
應用
角蛋白酶可对原体（EB）、GAG分子和细胞进行酶处理，用于研究 肺炎衣原体 菌株侵入宿主细胞过程中糖胺聚糖（GAG）的作用。[3]
生化／生理作用
物理性質
角蛋白酶活化 0.10% SDS、1.0%CTAB 和 EDTA。角蛋白酶可被吐温 ® 20、DMSO、异丙醇、甲醇和乙醇部分抑制。
單位定義
在 37℃、pH 7.5 时， 1 个单位的酶每分钟水解酪蛋白，产生的吸光度值相当于 1 μmol (181 μg) 的酪氨酸 的Folin-Ciocalteau 试剂。
準備報告
该酶可溶于无菌水或磷酸盐缓冲液中，浓度为 0.5-1.0 mg/mL。新鲜制备的溶液的活性最好。但是，单次使用的角蛋白酶溶液等分试样可在-20℃ 下储存。
基底
角蛋白。角蛋白酶也被用于朊病毒和朊病毒样蛋白的降解。
其他說明
This enzyme contains a C-terminus 6-Histidine tag.
法律資訊
TWEEN is a registered trademark of Croda International PLC
訊號詞
Warning
危險分類
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
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