Before lyophilyzation, ficoll & sucrose are added stabilizers & the hemoglobin is dialyzed against 25 mM ammonium bicarbonate containing 0.1 mM EDTA. It is expected that after lyophilization, there should be virtually no or substantially reduced ammonium bicarbonate. Stabilizers are the major components of the final product.
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一般說明
血红蛋白是红细胞的主要成分，也是红细胞呈现红色的原因。它在红细胞中的正常浓度为34％。由于它能够将氧从肺部输送到身体组织，促进二氧化碳的返回转运，因此血红蛋白是脊椎动物最重要的呼吸蛋白。
has not been tested for functional equivalence against native preparations (unlyophilized ferrous hemoglobins).
應用
亚铁稳定化人血红蛋白A0可用于：
生化／生理作用
血红蛋白（Hb）编码基因的变异会导致血红蛋白病（如地中海贫血）和非典型结构变异（如Hb Lepore和HbE）。[5]
包裝
包装规格标示出通过 Drabkin, D.L., J. Biol. Chem., 164, 703 (1946) 中介绍的方法测定的血红蛋白含量。
重構
用缓冲液复原时，可得到 >85% 的亚铁血红蛋白。
免責聲明
供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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From which buffer was Product H0267 lyophilized? Did this buffer contain primary amines?
1 answer-
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Does the product, H0267, contain HbA1c?
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The glycation level of this product, i.e., HbA1c, has not been characterized.
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How can Product H0267, Hemoglobin A0, be dissolved?
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Hemoglobin is soluble in water at 20 mg/mL. Solutions in phosphate buffer at about neutral pH can also be prepared.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the solution stability of Product H0267, Hemoglobin A0?
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Solutions of ferrous hemoglobin are not stable indefinitely due to the likely autoxidation of the heme group leading to the formation of the ferric derivative (methemoglobin). It is recommended to prepare solutions fresh in deaerated water or buffer. If necessary, store such prepared solutions no more than 1-2 days at 2-8°C.
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What is the structure of Product H0267, Hemoglobin A0?
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Hemoglobin A0 is the major hemoglobin protein of adult hemoglobin. It is a tetrameric protein consisting of 2 alpha and 2 beta chains, each of which contains a heme moiety and iron.
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Is there methemoglobin in Product H0267, Hemoglobin A0?
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The product may contain a small amount of methemoglobin as described on the specification sheet.
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Can Product H0267, Hemoglobin A0, be used in functional studies involving the binding and release of oxygen as a ligand?
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Sigma has not validated this hemoglobin for use in functional studies. The iron in the heme moiety is in the +2 state. However, this does not mean that this hemoglobin will behave necessarily like native hemoglobin nor be useful for functional studies of the molecule.
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What is the molecular weight of H0267 Hemoglobin A0?
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Sigma has not evaluated the molecular weight of hemoglobin. Based on the reported sequence and including the weight of the 4 heme groups and 4 iron atoms, the molecular weight is approximately 64,677 Daltons. The Structure and Action of Proteins, p. 52, Harper and Row (1969).
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