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重要文件

M0630

Sigma-Aldrich

肌红蛋白 来源于马骨骼肌

95-100%, essentially salt-free, lyophilized powder

同義詞:

肌红蛋白 来源于马骨骼肌

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.61
暫時無法取得訂價和供貨情況

生物源

equine skeletal muscle

化驗

95-100%

形狀

essentially salt-free, lyophilized powder

分子量

~17 kDa(lit.)

鐵含量

0.25-0.32%

技術

mass spectrometry (MS): suitable

溶解度

H2O: soluble 10 mg/mL

UniProt登錄號

儲存溫度

−20°C

基因資訊

horse ... MB(100054434)

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應用

马骨骼肌肌红蛋白被应用于一项对实验蛋白混合物进行质谱分析的研究中。[1]

生化/生理作用

肌红蛋白对骨骼肌在接近最大氧气需求时的O2 的供应至关重要,并通过将PO2 维持在高于支持线粒体功能所需的水平来防止缺氧。
马骨骼肌肌红蛋白是一种单链血红素蛋白,含有153个氨基酸残基。[2]它没有二硫键或游离-SH基团。肌红蛋白含8个大小不一的右旋螺旋区域,并由无序或随机线圈区域连接。[3]

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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L Henry et al.
Structural dynamics (Melville, N.Y.), 7(5), 054702-054702 (2020-09-29)
The correct folding of proteins is of paramount importance for their function, and protein misfolding is believed to be the primary cause of a wide range of diseases. Protein folding has been investigated with time-averaged methods and time-resolved spectroscopy, but
Emily S Choy et al.
The Journal of experimental biology, 222(Pt 11) (2019-05-18)
Arctic marine ecosystems are currently undergoing rapid environmental changes. Over the past 20 years, individual growth rates of beluga whales (Delphinapterus leucas) have declined, which may be a response to climate change; however, the scarcity of physiological data makes it difficult
Anthony W Maresso et al.
PLoS pathogens, 4(8), e1000132-e1000132 (2008-08-30)
Acquisition of iron is necessary for the replication of nearly all bacterial pathogens; however, iron of vertebrate hosts is mostly sequestered by heme and bound to hemoglobin within red blood cells. In Bacillus anthracis, the spore-forming agent of anthrax, the
R A Masaracchia et al.
The Journal of parasitology, 72(2), 299-305 (1986-04-01)
Calmodulin was purified from the obliquely striated skeletal muscle of Ascaris suum. The calmodulin had a molecular weight of 16,400 and the amino acid composition indicated it is highly similar to other purified calmodulins, showing insignificant variation in 12 of
The absorption spectra and extinction coefficients of myoglobin.
W J BOWEN
The Journal of biological chemistry, 179(1), 235-245 (1949-05-01)

文章

Professors summarize recent 2D materials synthesis advancements and biosensing applications in various fields.

Chromatograms

application for HPLC

Questions

1–4 of 4 Questions  
  1. Do you know the iron content in total for this product (1g)?

    1 answer
    1. The iron content for this product ranges from 0.25% to 0.32%. The specific iron content is lot-dependent and can be found in the Certificate of Analysis.

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  2. Can you advise what the % level of hemoglobin (if any will be present in this product)? Or can this be treated as Hb contaminant free.

    1 answer
    1. While this product is not specifically tested for hemoglobin, it is purified by Sephadex gel filtration. Given the molecular weight differences between these two compounds, the presence of hemoglobin is highly unlikely.

      Helpful?

  3. Can you share the amino acid sequence of this protein? M0630

    1 answer
    1. This product is a native protein that has not been modified during the extraction and purification process. The sequence of this product is published information. Please refer to the information in the UniProt database: https://www.uniprot.org/uniprotkb/P68082/entry

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  4. Is this myoglobin detectable using immunoenzymatic assay?

    1 answer
    1. Unfortunately, none of the equine myoglobin products have been tested for suitability in ELISA. Performance of these products in immunoenzymatic assays cannot be validated.

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