推薦產品
ligand
alkali-stabilized protein A-derived (E. coli)
包裝
pack of 1 × 5 mL
製造商／商標名
Cytiva 11-0034-94
儲存條件
(20% Ehtanol)
(20% Ethanol)
參數
<20 mL/min flow rate
42 psi
床尺寸
16 mm × 25 mm
床體積
5 mL
柱內徑
16 mm
基質
highly cross-linked agarose
樣品平均尺寸
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-13.7
工作範圍
3-12
容量
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
適合性
suitable for bioprocess medium
儲存溫度
2-8°C
尋找類似的產品？ 前往 產品比較指南
相關類別
一般說明
Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect™ SuRe™ columns are prepacked with MabSelect™ SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).
HiTrap® MabSelect™ columns are prepacked with MabSelect™ a bioprocess medium for capuring of Mabs from large sample volumes.
HiTrap® MabSelect™ columns are prepacked with MabSelect™ a bioprocess medium for capuring of Mabs from large sample volumes.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
特點和優勢
- Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
- Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Prepacked HiTrap® columns for process development, screening of purification conditions, and small-scale purification of Mabs.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
其他說明
1)The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
法律資訊
Free MabSelect SuRe Ligand
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva
MabSelect SuRe is a trademark of Cytiva
SuRe is a trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
客戶也查看了
文章
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
在實驗室規模下進行脫鹽是一種行之有效、簡單且快速的方法，可快速去除低分子量雜質，同時只需一個步驟即可將樣品轉移到所需的緩衝液中。
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務
